气候变化领域集成服务门户(CCPortal): Massively multiplex chemical transcriptomics at single-cell resolution

CCPortal

DOI	10.1126/science.aax6234
	Massively multiplex chemical transcriptomics at single-cell resolution
	Srivatsan S.R.; McFaline-Figueroa J.L.; Ramani V.; Saunders L.; Cao J.; Packer J.; Pliner H.A.; Jackson D.L.; Daza R.M.; Christiansen L.; Zhang F.; Steemers F.; Shendure J.; Trapnell C.
发表日期	2020
ISSN	0036-8075
起始页码	45
结束页码	51
卷号	367 期号:6473
英文摘要	High-throughput chemical screens typically use coarse assays such as cell survival, limiting what can be learned about mechanisms of action, off-target effects, and heterogeneous responses. Here, we introduce “sci-Plex,” which uses “nuclear hashing” to quantify global transcriptional responses to thousands of independent perturbations at single-cell resolution. As a proof of concept, we applied sci-Plex to screen three cancer cell lines exposed to 188 compounds. In total, we profiled ~650,000 single-cell transcriptomes across ~5000 independent samples in one experiment. Our results reveal substantial intercellular heterogeneity in response to specific compounds, commonalities in response to families of compounds, and insight into differential properties within families. In particular, our results with histone deacetylase inhibitors support the view that chromatin acts as an important reservoir of acetate in cancer cells. © 2020 American Association for the Advancement of Science. All rights reserved.
关键词	4 (2 aminoethylamino) 1,8 dimethylimidazo[1,2 a]quinoxaline abexinostat acetic acid belinostat cudc 101 fimepinostat givinostat histone deacetylase inhibitor m 344 n [2 [3 (1 piperazinylmethyl)imidazo[2,1 b]thiazol 6 yl]phenyl] 2 quinoxalinecarboxamide panobinostat pracinostat rec 2282 trametinib transcriptome trichostatin A unclassified drug transcriptome cancer cell chemical analysis detection method experimental study genetic analysis resolution A-549 cell line animal cell animal experiment Article cell function cell heterogeneity cell labeling cell proliferation chromatin controlled study dose response drug activity experimentation gene control genetic transcription human human cell K-562 cell line MCF-7 cell line mouse nonhuman pilot study priority journal proof of concept RNA sequence screening single cell analysis transcriptomics cell nucleus drug effect genetics genomics high throughput screening metabolism neoplasm procedures single cell analysis A549 Cells Acetates Cell Nucleus Chromatin Genomics High-Throughput Screening Assays Histone Deacetylase Inhibitors Humans K562 Cells MCF-7 Cells Neoplasms RNA-Seq Single-Cell Analysis Transcriptome
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133804
推荐引用方式 GB/T 7714	Srivatsan S.R.,McFaline-Figueroa J.L.,Ramani V.,et al. Massively multiplex chemical transcriptomics at single-cell resolution[J]. Science,2020,367(6473).
APA	Srivatsan S.R..,McFaline-Figueroa J.L..,Ramani V..,Saunders L..,Cao J..,...&Trapnell C..(2020).Massively multiplex chemical transcriptomics at single-cell resolution.,367(6473).
MLA	Srivatsan S.R.,et al."Massively multiplex chemical transcriptomics at single-cell resolution".367.6473(2020).