气候变化领域集成服务门户(CCPortal): High Antioxidant Capacity of Lacticaseibacillus paracasei TDM-2 and Pediococcus pentosaceus TCM-3 from Qinghai Tibetan Plateau and Their Function towards Gut Modulation

CCPortal

DOI	10.3390/foods12091814
	High Antioxidant Capacity of Lacticaseibacillus paracasei TDM-2 and Pediococcus pentosaceus TCM-3 from Qinghai Tibetan Plateau and Their Function towards Gut Modulation
	Yang, Tingyu; Fan, Xueni; Li, Diantong; Zhao, Tingting; Wu, Dan; Liu, Zhenjiang; Long, Danfeng; Li, Bin; Huang, Xiaodan
发表日期	2023
EISSN	2304-8158
卷号	12 期号:9
英文摘要	Probiotic supplementation is a key therapeutic strategy for promoting gut health and maintaining gut homeostasis by modulating functional microbiota. In this study, we isolated two lactic acid bacteria (LAB) strains, Pediococcus pentosaceus TCM-3 and Lacticaseibacillus paracasei TDM-2, from Qinghai-Tibetan plateau, and evaluated their probiotic properties and antioxidant bioactivity. In which, TDM-2 had higher T-AOC activity than either TCM-3 or LGG (4.10 mu mol/mL vs. 3.68 and 3.53 mu mol/mL, respectively, p < 0.05). These strains have shown high antioxidant activity compared to the LAB strains and were found to be acid and bile salt tolerant, confronting the safety issues of antibiotic resistance and the capability of surviving in simulated gastric and intestinal juices. In vitro fermentation experiments with human gut microbiota revealed significant differences in microbial community composition between samples supplemented with TCM-3 and TDM-2 and those without. The addition of these two strains resulted in an enrichment of beneficial taxa, such as the Pediococcus, Lactobacillus, and Clostridium_sensu_strictos at the genus level, and Firmicutes and Proteobacteria at the phylum level. Notably, the TCM-3 group exhibited higher short-chain fatty acid production than the TDM-2 group and untreated controls (acetic acid at 12 h: 4.54 mmol L-1 vs. 4.06 mmol L-1 and 4.00 mmol L-1; acetic acid at 24 h: 4.99 mmol L-1 vs. 4.90 mmol L-1 and 4.82 mmol L-1, p < 0.05). These findings demonstrate that LAB supplementation with high antioxidant capacity and probiotic properties can promote gut health by modulating functional microbiota and is enriching for beneficial taxa. Our study provides guidance for therapeutic strategies that use novel LAB strains to maintain gut homeostasis and functional microbiota modulation.
关键词	lactic acid bacteria Qinghai-Tibetan plateau antioxidant capacity gut microbiota
英文关键词	LACTIC-ACID BACTERIA; OXIDATIVE STRESS; IN-VITRO; LACTOBACILLUS; STRAINS; ADHESION; MECHANISMS; MICROBIOTA; DIVERSITY; RESPONSES
WOS研究方向	Food Science & Technology
WOS记录号	WOS:000987201300001
来源期刊	FOODS
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/283596
作者单位	Lanzhou University; Ministry of Agriculture & Rural Affairs; Tibet Academy of Agriculture & Animal Husbandry Sciences; Jilin University
推荐引用方式 GB/T 7714	Yang, Tingyu,Fan, Xueni,Li, Diantong,et al. High Antioxidant Capacity of Lacticaseibacillus paracasei TDM-2 and Pediococcus pentosaceus TCM-3 from Qinghai Tibetan Plateau and Their Function towards Gut Modulation[J],2023,12(9).
APA	Yang, Tingyu.,Fan, Xueni.,Li, Diantong.,Zhao, Tingting.,Wu, Dan.,...&Huang, Xiaodan.(2023).High Antioxidant Capacity of Lacticaseibacillus paracasei TDM-2 and Pediococcus pentosaceus TCM-3 from Qinghai Tibetan Plateau and Their Function towards Gut Modulation.FOODS,12(9).
MLA	Yang, Tingyu,et al."High Antioxidant Capacity of Lacticaseibacillus paracasei TDM-2 and Pediococcus pentosaceus TCM-3 from Qinghai Tibetan Plateau and Their Function towards Gut Modulation".FOODS 12.9(2023).