气候变化领域集成服务门户(CCPortal): Temperature-dependent sex determination is mediated by pSTAT3 repression of Kdm6b

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DOI	10.1126/science.aaz4165
	Temperature-dependent sex determination is mediated by pSTAT3 repression of Kdm6b
	Weber C.; Zhou Y.; Lee J.G.; Looger L.L.; Qian G.; Ge C.; Capel B.
发表日期	2020
ISSN	0036-8075
起始页码	303
结束页码	306
卷号	368 期号:6488
英文摘要	In many reptiles, including the red-eared slider turtle Trachemys scripta elegans (T. scripta), sex is determined by ambient temperature during embryogenesis. We previously showed that the epigenetic regulator Kdm6b is elevated at the male-producing temperature and essential to activate the male pathway. In this work, we established a causal link between temperature and transcriptional regulation of Kdm6b. We show that signal transducer and activator of transcription 3 (STAT3) is phosphorylated at the warmer, female-producing temperature, binds the Kdm6b locus, and represses Kdm6b transcription, blocking the male pathway. Influx of Ca2+, a mediator of STAT3 phosphorylation, is elevated at the female temperature and acts as a temperature-sensitive regulator of STAT3 activation. © 2020 American Association for the Advancement of Science. All rights reserved.
英文关键词	STAT3 protein; calcium; histone demethylase; STAT3 protein; embryonic development; gene expression; sex determination; temperature effect; turtle; animal cell; animal experiment; Article; binding site; calcium transport; cell activation; chromatin immunoprecipitation; controlled study; dimerization; Dmrt1 gene; embryo; embryo development; environmental temperature; epigenetic repression; female; gene; gene activation; gene interaction; gene targeting; genetic transcription; gonad; high temperature; immunofluorescence test; Kdm6b gene; male; nonhuman; priority journal; protein phosphorylation; real time polymerase chain reaction; sex determination; signal transduction; temperature dependence; thermoregulation; transcription regulation; upregulation; Western blotting; animal; embryology; gene expression regulation; genetics; metabolism; phosphorylation; sex determination process; temperature; turtle; Reptilia; Trachemys scripta; Trachemys scripta elegans; Animals; Calcium; Female; Gene Expression Regulation, Developmental; Jumonji Domain-Containing Histone Demethylases; Male; Phosphorylation; Sex Determination Processes; STAT3 Transcription Factor; Temperature; Transcription, Genetic; Turtles
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/245982
作者单位	Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, United States; College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, 315100, China; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, United States
推荐引用方式 GB/T 7714	Weber C.,Zhou Y.,Lee J.G.,et al. Temperature-dependent sex determination is mediated by pSTAT3 repression of Kdm6b[J],2020,368(6488).
APA	Weber C..,Zhou Y..,Lee J.G..,Looger L.L..,Qian G..,...&Capel B..(2020).Temperature-dependent sex determination is mediated by pSTAT3 repression of Kdm6b.Science,368(6488).
MLA	Weber C.,et al."Temperature-dependent sex determination is mediated by pSTAT3 repression of Kdm6b".Science 368.6488(2020).