气候变化领域集成服务门户(CCPortal): SARS-CoV-2 nucleocapsid protein phase separates with G3BPs to disassemble stress granules and facilitate viral production

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DOI	10.1016/j.scib.2021.01.013
	SARS-CoV-2 nucleocapsid protein phase separates with G3BPs to disassemble stress granules and facilitate viral production
	Luo L.; Li Z.; Zhao T.; Ju X.; Ma P.; Jin B.; Zhou Y.; He S.; Huang J.; Xu X.; Zou Y.; Li P.; Liang A.; Liu J.; Chi T.; Huang X.; Ding Q.; Jin Z.; Huang C.; Zhang Y.
发表日期	2021
ISSN	20959273
起始页码	1194
结束页码	1204
卷号	66 期号:12
英文摘要	A key to tackling the coronavirus disease 2019 (COVID-19) pandemic is to understand how severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) manages to outsmart host antiviral defense mechanisms. Stress granules (SGs), which are assembled during viral infection and function to sequester host and viral mRNAs and proteins, are part of the antiviral responses. Here, we show that the SARS-CoV-2 nucleocapsid (N) protein, an RNA binding protein essential for viral production, interacted with Ras-GTPase-activating protein SH3-domain-binding protein (G3BP) and disrupted SG assembly, both of which require intrinsically disordered region1 (IDR1) in N protein. The N protein partitioned into SGs through liquid-liquid phase separation with G3BP, and blocked the interaction of G3BP1 with other SG-related proteins. Moreover, the N protein domains important for phase separation with G3BP and SG disassembly were required for SARS-CoV-2 viral production. We propose that N protein-mediated SG disassembly is crucial for SARS-CoV-2 production. © 2021 Science China Press
关键词	G3BP Liquid-liquid phase separation Nucleocapsid protein SARS-CoV-2 Stress granules Viral production
英文关键词	Diseases; Granulation; Liquids; Proteins; Antivirals; Coronaviruses; G3BP; Liquid-liquid phase separation; Nucleocapsid proteins; Protein phasis; Severe acute respiratory syndrome coronavirus; Severe acute respiratory syndrome coronavirus 2; Stress granule; Viral production; Phase separation
语种	英语
来源期刊	Science Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/207516
作者单位	School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China; Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, 201210, China; College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, 321004, China; Center for Infectious Disease Research, School of Medicine, Tsinghua University, Beijing, 100084, China; Department of Hematology, Tongji Hospital of Tongji University, Shanghai, 200065, China; Beijing Advanced Innovation Center for Structural Biology, Tsinghua University, Beijing, 100084, China
推荐引用方式 GB/T 7714	Luo L.,Li Z.,Zhao T.,et al. SARS-CoV-2 nucleocapsid protein phase separates with G3BPs to disassemble stress granules and facilitate viral production[J],2021,66(12).
APA	Luo L..,Li Z..,Zhao T..,Ju X..,Ma P..,...&Zhang Y..(2021).SARS-CoV-2 nucleocapsid protein phase separates with G3BPs to disassemble stress granules and facilitate viral production.Science Bulletin,66(12).
MLA	Luo L.,et al."SARS-CoV-2 nucleocapsid protein phase separates with G3BPs to disassemble stress granules and facilitate viral production".Science Bulletin 66.12(2021).