气候变化领域集成服务门户(CCPortal): Lipid-gated monovalent ion fluxes regulate endocytic traffic and support immune surveillance

CCPortal

DOI	10.1126/science.aaw9544
	Lipid-gated monovalent ion fluxes regulate endocytic traffic and support immune surveillance
	Freeman S.A.; Uderhardt S.; Saric A.; Collins R.F.; Buckley C.M.; Mylvaganam S.; Boroumand P.; Plumb J.; Germain R.N.; Ren D.; Grinstein S.
发表日期	2020
ISSN	0036-8075
起始页码	301
结束页码	305
卷号	367 期号:6475
英文摘要	Despite ongoing (macro)pinocytosis of extracellular fluid, the volume of the endocytic pathway remains unchanged. To investigate the underlying mechanism, we used high-resolution video imaging to analyze the fate of macropinosomes formed by macrophages in vitro and in situ. Na+, the primary cationic osmolyte internalized, exited endocytic vacuoles via two-pore channels, accompanied by parallel efflux of Cl− and osmotically coupled water. The resulting shrinkage caused crenation of the membrane, which fostered recruitment of curvature-sensing proteins. These proteins stabilized tubules and promoted their elongation, driving vacuolar remodeling, receptor recycling, and resolution of the organelles. Failure to resolve internalized fluid impairs the tissue surveillance activity of resident macrophages. Thus, osmotically driven increases in the surface-to-volume ratio of endomembranes promote traffic between compartments and help to ensure tissue homeostasis. © 2020 American Association for the Advancement of Science. All rights reserved.
关键词	cation channel chemokine receptor CCR2 chloride colony stimulating factor 1 epidermal growth factor epidermal growth factor receptor lipid sialoadhesin sodium ion tetrandrine two pore channel unclassified drug calcium channel lipid Mcoln1 protein, mouse sodium TPCN1 protein, mouse TPCN2 protein, mouse transient receptor potential channel cation homeostasis immune system protein recycling animal experiment Article bone marrow derived macrophage cell vacuole cell volume channel gating chloride transport concentration (parameter)controlled study endocytosis extracellular fluid extracellular space homeostasis human human cell human tissue immunosurveillance in vitro study internalization ion current knockout mouse macropinocytosis mouse nonhuman priority journal protein stability RNA interference sodium transport tonoplast animal cell organelle endosome genetics immunology ion transport macrophage metabolism osmosis physiology pinocytosis Animals Calcium Channels Endosomes Immunologic Surveillance Ion Transport Lipids Macrophages Mice Mice, Knockout Organelles Osmosis Pinocytosis Sodium Transient Receptor Potential Channels Vacuoles
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133773
推荐引用方式 GB/T 7714	Freeman S.A.,Uderhardt S.,Saric A.,et al. Lipid-gated monovalent ion fluxes regulate endocytic traffic and support immune surveillance[J]. Science,2020,367(6475).
APA	Freeman S.A..,Uderhardt S..,Saric A..,Collins R.F..,Buckley C.M..,...&Grinstein S..(2020).Lipid-gated monovalent ion fluxes regulate endocytic traffic and support immune surveillance.,367(6475).
MLA	Freeman S.A.,et al."Lipid-gated monovalent ion fluxes regulate endocytic traffic and support immune surveillance".367.6475(2020).