气候变化领域集成服务门户(CCPortal): Valence and patterning of aromatic residues determine the phase behavior of prion-like domains

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DOI	10.1126/science.aaw8653
	Valence and patterning of aromatic residues determine the phase behavior of prion-like domains
	Martin E.W.; Holehouse A.S.; Peran I.; Farag M.; Incicco J.J.; Bremer A.; Grace C.R.; Soranno A.; Pappu R.V.; Mittag T.
发表日期	2020
ISSN	0036-8075
起始页码	694
结束页码	699
卷号	367 期号:6478
英文摘要	Prion-like domains (PLDs) can drive liquid-liquid phase separation (LLPS) in cells. Using an integrative biophysical approach that includes nuclear magnetic resonance spectroscopy, small-angle x-ray scattering, and multiscale simulations, we have uncovered sequence features that determine the overall phase behavior of PLDs. We show that the numbers (valence) of aromatic residues in PLDs determine the extent of temperature-dependent compaction of individual molecules in dilute solutions. The valence of aromatic residues also determines full binodals that quantify concentrations of PLDs within coexisting dilute and dense phases as a function of temperature. We also show that uniform patterning of aromatic residues is a sequence feature that promotes LLPS while inhibiting aggregation. Our findings lead to the development of a numerical stickers-and-spacers model that enables predictions of full binodals of PLDs from their sequences. © 2020 American Association for the Advancement of Science. All rights reserved.
关键词	aromatic hydrocarbon biophysics concentration (composition)genetic analysis nuclear magnetic resonance prion disease protein separation temperature effect article nonhuman nuclear magnetic resonance spectroscopy phase separation prediction prion simulation X ray crystallography amino acid sequence chemistry phase transition prion protein domain small angle scattering X ray diffraction heterogeneous nuclear ribonucleoprotein A1 phenylalanine tyrosine Amino Acid Sequence Heterogeneous Nuclear Ribonucleoprotein A1 Magnetic Resonance Spectroscopy Phase Transition Phenylalanine Prions Protein Domains Scattering, Small Angle Tyrosine X-Ray Diffraction
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133745
推荐引用方式 GB/T 7714	Martin E.W.,Holehouse A.S.,Peran I.,et al. Valence and patterning of aromatic residues determine the phase behavior of prion-like domains[J]. Science,2020,367(6478).
APA	Martin E.W..,Holehouse A.S..,Peran I..,Farag M..,Incicco J.J..,...&Mittag T..(2020).Valence and patterning of aromatic residues determine the phase behavior of prion-like domains.,367(6478).
MLA	Martin E.W.,et al."Valence and patterning of aromatic residues determine the phase behavior of prion-like domains".367.6478(2020).