气候变化领域集成服务门户(CCPortal): Lineage tracing on transcriptional landscapes links state to fate during differentiation

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DOI	10.1126/science.aaw3381
	Lineage tracing on transcriptional landscapes links state to fate during differentiation
	Weinreb C.; Rodriguez-Fraticelli A.; Camargo F.D.; Klein A.M.
发表日期	2020
ISSN	0036-8075
卷号	367 期号:6479
英文摘要	A challenge in biology is to associate molecular differences among progenitor cells with their capacity to generate mature cell types. Here, we used expressed DNA barcodes to clonally trace transcriptomes over time and applied this to study fate determination in hematopoiesis. We identified states of primed fate potential and located them on a continuous transcriptional landscape. We identified two routes of monocyte differentiation that leave an imprint on mature cells. Analysis of sister cells also revealed cells to have intrinsic fate biases not detectable by single-cell RNA sequencing. Finally, we benchmarked computational methods of dynamic inference from single-cell snapshots, showing that fate choice occurs earlier than is detected by state-of the-art algorithms and that cells progress steadily through pseudotime with precise and consistent dynamics. © 2020 American Association for the Advancement of Science. All rights reserved.
关键词	biology cell component differentiation DNA RNA transcriptome algorithm animal cell lineage cytology DNA barcoding gene expression genetics hematopoiesis monocyte mouse procedures single cell analysis Algorithms Animals Cell Lineage DNA Barcoding, Taxonomic Gene Expression Hematopoiesis Mice Monocytes RNA-Seq Single-Cell Analysis Transcriptome
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133705
推荐引用方式 GB/T 7714	Weinreb C.,Rodriguez-Fraticelli A.,Camargo F.D.,et al. Lineage tracing on transcriptional landscapes links state to fate during differentiation[J]. Science,2020,367(6479).
APA	Weinreb C.,Rodriguez-Fraticelli A.,Camargo F.D.,&Klein A.M..(2020).Lineage tracing on transcriptional landscapes links state to fate during differentiation.,367(6479).
MLA	Weinreb C.,et al."Lineage tracing on transcriptional landscapes links state to fate during differentiation".367.6479(2020).