气候变化领域集成服务门户(CCPortal): Temperature-dependent sex determination is mediated by pSTAT3 repression of Kdm6b

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DOI	10.1126/science.aaz4165
	Temperature-dependent sex determination is mediated by pSTAT3 repression of Kdm6b
	Weber C.; Zhou Y.; Lee J.G.; Looger L.L.; Qian G.; Ge C.; Capel B.
发表日期	2020
ISSN	0036-8075
起始页码	303
结束页码	306
卷号	368 期号:6488
英文摘要	In many reptiles, including the red-eared slider turtle Trachemys scripta elegans (T. scripta), sex is determined by ambient temperature during embryogenesis. We previously showed that the epigenetic regulator Kdm6b is elevated at the male-producing temperature and essential to activate the male pathway. In this work, we established a causal link between temperature and transcriptional regulation of Kdm6b. We show that signal transducer and activator of transcription 3 (STAT3) is phosphorylated at the warmer, female-producing temperature, binds the Kdm6b locus, and represses Kdm6b transcription, blocking the male pathway. Influx of Ca2+, a mediator of STAT3 phosphorylation, is elevated at the female temperature and acts as a temperature-sensitive regulator of STAT3 activation. © 2020 American Association for the Advancement of Science. All rights reserved.
关键词	STAT3 protein calcium histone demethylase STAT3 protein embryonic development gene expression sex determination temperature effect turtle animal cell animal experiment Article binding site calcium transport cell activation chromatin immunoprecipitation controlled study dimerization Dmrt1 gene embryo embryo development environmental temperature epigenetic repression female gene gene activation gene interaction gene targeting genetic transcription gonad high temperature immunofluorescence test Kdm6b gene male nonhuman priority journal protein phosphorylation real time polymerase chain reaction sex determination signal transduction temperature dependence thermoregulation transcription regulation upregulation Western blotting animal embryology gene expression regulation genetics metabolism phosphorylation sex determination process temperature turtle Reptilia Trachemys scripta Trachemys scripta elegans Animals Calcium Female Gene Expression Regulation, Developmental Jumonji Domain-Containing Histone Demethylases Male Phosphorylation Sex Determination Processes STAT3 Transcription Factor Temperature Transcription, Genetic Turtles
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133563
推荐引用方式 GB/T 7714	Weber C.,Zhou Y.,Lee J.G.,et al. Temperature-dependent sex determination is mediated by pSTAT3 repression of Kdm6b[J]. Science,2020,368(6488).
APA	Weber C..,Zhou Y..,Lee J.G..,Looger L.L..,Qian G..,...&Capel B..(2020).Temperature-dependent sex determination is mediated by pSTAT3 repression of Kdm6b.,368(6488).
MLA	Weber C.,et al."Temperature-dependent sex determination is mediated by pSTAT3 repression of Kdm6b".368.6488(2020).