气候变化领域集成服务门户(CCPortal): Metabolomics and mass spectrometry imaging reveal channeled de novo purine synthesis in cells

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DOI	10.1126/science.aaz6465
	Metabolomics and mass spectrometry imaging reveal channeled de novo purine synthesis in cells
	Pareek V.; Tian H.; Winograd N.; Benkovic S.J.
发表日期	2020
ISSN	0036-8075
起始页码	283
结束页码	290
卷号	368 期号:6488
英文摘要	Metabolons, multiprotein complexes consisting of sequential enzymes of a metabolic pathway, are proposed to be biosynthetic "hotspots" within the cell. However, experimental demonstration of their presence and functions has remained challenging. We used metabolomics and in situ three-dimensional submicrometer chemical imaging of single cells by gas cluster ion beam secondary ion mass spectrometry (GCIB-SIMS) to directly visualize de novo purine biosynthesis by a multienzyme complex, the purinosome. We found that purinosomes comprise nine enzymes that act synergistically, channeling the pathway intermediates to synthesize purine nucleotides, increasing the pathway flux, and influencing the adenosine monophosphate/guanosine monophosphate ratio. Our work also highlights the application of high-resolution GCIB-SIMS for multiplexed biomolecular analysis at the level of single cells. © 2020 American Association for the Advancement of Science. All rights reserved.
关键词	adenosine phosphate guanosine phosphate multienzyme complex nucleotide purine purine purine derivative cell experimental study imaging method mass spectrometry metabolism protein Article biochemistry enzyme assay gas cluster ion beam secondary ion mass spectrometry image analysis mass spectrometry metabolomics mitochondrion molecular imaging molecular probe priority journal purine synthesis reaction analysis single cell analysis biosynthesis fluorescence imaging HeLa cell line human metabolism metabolomics procedures secondary ion mass spectrometry HeLa Cells Humans Metabolomics Mitochondria Multienzyme Complexes Optical Imaging Purines Single-Cell Analysis Spectrometry, Mass, Secondary Ion
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133558
推荐引用方式 GB/T 7714	Pareek V.,Tian H.,Winograd N.,et al. Metabolomics and mass spectrometry imaging reveal channeled de novo purine synthesis in cells[J]. Science,2020,368(6488).
APA	Pareek V.,Tian H.,Winograd N.,&Benkovic S.J..(2020).Metabolomics and mass spectrometry imaging reveal channeled de novo purine synthesis in cells.,368(6488).
MLA	Pareek V.,et al."Metabolomics and mass spectrometry imaging reveal channeled de novo purine synthesis in cells".368.6488(2020).