气候变化领域集成服务门户(CCPortal): Structural basis for transcriptional start site control of HIV-1 RNA fate

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DOI	10.1126/science.aaz7959
	Structural basis for transcriptional start site control of HIV-1 RNA fate
	Brown J.D.; Kharytonchyk S.; Chaudry I.; Iyer A.S.; Carter H.; Becker G.; Desai Y.; Glang L.; Choi S.H.; Singh K.; Lopresti M.W.; Orellana M.; Rodriguez T.; Oboh U.; Hijji J.; Ghinger F.G.; Stewart K.; Francis D.; Edwards B.; Chen P.; Case D.A.; Telesnitsky A.; Summers M.F.
发表日期	2020
ISSN	0036-8075
起始页码	413
结束页码	417
卷号	368 期号:6489
英文摘要	Heterogeneous transcriptional start site usage by HIV-1 produces 5′-capped RNAs beginning with one, two, or three 5′-guanosines (Cap1G, Cap2G, or Cap3G, respectively) that are either selected for packaging as genomes (Cap1G) or retained in cells as translatable messenger RNAs (mRNAs) (Cap2G and Cap3G). To understand how 5′-guanosine number influences fate, we probed the structures of capped HIV-1 leader RNAs by deuterium-edited nuclear magnetic resonance. The Cap1G transcript adopts a dimeric multihairpin structure that sequesters the cap, inhibits interactions with eukaryotic translation initiation factor 4E, and resists decapping. The Cap2G and Cap3G transcripts adopt an alternate structure with an elongated central helix, exposed splice donor residues, and an accessible cap. Extensive remodeling, achieved at the energetic cost of a G-C base pair, explains how a single 5′-guanosine modifies the function of a ~9-kilobase HIV-1 transcript. Copyright © 2020 The Authors,
关键词	capped RNA initiation factor 4E messenger RNA virus RNA capped RNA guanosine initiation factor 4E messenger RNA virus RNA cell chemical binding deuterium genome human immunodeficiency virus physiological response RNA Article base pairing deuteron nuclear magnetic resonance dimerization DNA helix energy transfer gene control gene function gene number human Human immunodeficiency virus 1 nonhuman priority journal promoter region RNA probe RNA structure RNA transcription virus replication 5' untranslated region base pairing chemistry DNA base composition gene expression regulation genetics Human immunodeficiency virus 1 metabolism nuclear magnetic resonance protein synthesis transcription initiation site Eukaryota Human immunodeficiency virus 1 5' Untranslated Regions Base Composition Base Pairing Eukaryotic Initiation Factor-4E Gene Expression Regulation, Viral Guanosine HIV-1 Humans Nuclear Magnetic Resonance, Biomolecular Protein Biosynthesis RNA Caps RNA, Messenger RNA, Viral Transcription Initiation Site
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133542
推荐引用方式 GB/T 7714	Brown J.D.,Kharytonchyk S.,Chaudry I.,et al. Structural basis for transcriptional start site control of HIV-1 RNA fate[J]. Science,2020,368(6489).
APA	Brown J.D..,Kharytonchyk S..,Chaudry I..,Iyer A.S..,Carter H..,...&Summers M.F..(2020).Structural basis for transcriptional start site control of HIV-1 RNA fate.,368(6489).
MLA	Brown J.D.,et al."Structural basis for transcriptional start site control of HIV-1 RNA fate".368.6489(2020).