气候变化领域集成服务门户(CCPortal): Regenerative potential of prostate luminal cells revealed by single-cell analysis

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DOI	10.1126/science.aay0267
	Regenerative potential of prostate luminal cells revealed by single-cell analysis
	Karthaus W.R.; Hofree M.; Choi D.; Linton E.L.; Turkekul M.; Bejnood A.; Carver B.; Gopalan A.; Abida W.; Laudone V.; Biton M.; Chaudhary O.; Xu T.; Masilionis I.; Manova K.; Mazutis L.; Pe’er D.; Regev A.; Sawyers C.L.
发表日期	2020
ISSN	0036-8075
起始页码	497
结束页码	505
卷号	368 期号:6490
英文摘要	Androgen deprivation is the cornerstone of prostate cancer treatment. It results in involution of the normal gland to ~90% of its original size because of the loss of luminal cells. The prostate regenerates when androgen is restored, a process postulated to involve stem cells. Using single-cel RNA sequencing, we identified a rare luminal population in the mouse prostate that expresses stemlike genes (Sca1+ and Psca+) and a large population of differentiated cells (Nkx3.1+, Pbsn+). In organoids and in mice, both populations contribute equally to prostate regeneration, partly through androgen-driven expression of growth factors (Nrg2, Rspo3) by mesenchymal cells acting in a paracrine fashion on luminal cells. Analysis of human prostate tissue revealed similar differentiated and stemlike luminal subpopulations that likewise acquire enhanced regenerative potential after androgen ablation. We propose that prostate regeneration is driven by nearly all persisting luminal cells, not just by rare stem cells. © 2020 American Association for the Advancement of Science. All rights reserved.
关键词	androgen growth factor Nrg2 growth factor Rspo3 growth factor unclassified drug androgen binding protein antiandrogen ataxin 1 Atxn1 protein, mouse glycosylphosphatidylinositol anchored protein homeodomain protein nerve growth factor Nkx3-1 protein, mouse Nrg2 protein, mouse probasin Psca protein, mouse R-spondin3 protein, mouse thrombospondin transcription factor tumor antigen tumor protein androgen cancer cell gene expression RNA rodent ablation therapy animal cell animal tissue Article cell differentiation cell population cell regeneration cells by body anatomy controlled study gene gene expression human luminal cell male mesenchyme cell mouse nonhuman paracrine signaling priority journal prostate cell prostate tissue protein expression Psca gene RNA sequencing Sca1 gene single cell analysis animal genetics mesenchymal stem cell metabolism organ size organoid physiology prostate prostate tumor regeneration sequence analysis single cell analysis Mus Androgen Antagonists Androgen-Binding Protein Androgens Animals Antigens, Neoplasm Ataxin-1 Cell Differentiation Gene Expression GPI-Linked Proteins Homeodomain Proteins Humans Male Mesenchymal Stem Cells Mice Neoplasm Proteins Nerve Growth Factors Organ Size Organoids Prostate Prostatic Neoplasms Regeneration Sequence Analysis, RNA Single-Cell Analysis Thrombospondins Transcription Factors
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133530
推荐引用方式 GB/T 7714	Karthaus W.R.,Hofree M.,Choi D.,et al. Regenerative potential of prostate luminal cells revealed by single-cell analysis[J]. Science,2020,368(6490).
APA	Karthaus W.R..,Hofree M..,Choi D..,Linton E.L..,Turkekul M..,...&Sawyers C.L..(2020).Regenerative potential of prostate luminal cells revealed by single-cell analysis.,368(6490).
MLA	Karthaus W.R.,et al."Regenerative potential of prostate luminal cells revealed by single-cell analysis".368.6490(2020).