气候变化领域集成服务门户(CCPortal): Ion transport and regulation in a synaptic vesicle glutamate transporter

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DOI	10.1126/science.aba9202
	Ion transport and regulation in a synaptic vesicle glutamate transporter
	Li F.; Eriksen J.; Finer-Moore J.; Chang R.; Nguyen P.; Bowen A.; Myasnikov A.; Yu Z.; Bulkley D.; Cheng Y.; Edwards R.H.; Stroud R.M.
发表日期	2020
ISSN	0036-8075
起始页码	893
结束页码	897
卷号	368 期号:6493
英文摘要	Synaptic vesicles accumulate neurotransmitters, enabling the quantal release by exocytosis that underlies synaptic transmission. Specific neurotransmitter transporters are responsible for this activity and therefore are essential for brain function. The vesicular glutamate transporters (VGLUTs) concentrate the principal excitatory neurotransmitter glutamate into synaptic vesicles, driven by membrane potential. However, the mechanism by which they do so remains poorly understood owing to a lack of structural information. We report the cryo–electron microscopy structure of rat VGLUT2 at 3.8-angstrom resolution and propose structure-based mechanisms for substrate recognition and allosteric activation by low pH and chloride. A potential permeation pathway for chloride intersects with the glutamate binding site. These results demonstrate how the activity of VGLUTs can be coordinated with large shifts in proton and chloride concentrations during the synaptic vesicle cycle to ensure normal synaptic transmission. Copyright © 2020 The Authors,
关键词	brain chemical binding chloride neurology substrate Rattus chloride chloride channel glutamic acid Slc17a6 protein, rat vesicular glutamate transporter 2 allosterism amino acid sequence animal binding site chemistry cryoelectron microscopy ion transport membrane potential metabolism pH protein domain rat synapse vesicle Allosteric Regulation Amino Acid Sequence Animals Binding Sites Chloride Channels Chlorides Cryoelectron Microscopy Glutamic Acid Hydrogen-Ion Concentration Ion Transport Membrane Potentials Protein Domains Rats Synaptic Vesicles Vesicular Glutamate Transport Protein 2
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133479
推荐引用方式 GB/T 7714	Li F.,Eriksen J.,Finer-Moore J.,et al. Ion transport and regulation in a synaptic vesicle glutamate transporter[J]. Science,2020,368(6493).
APA	Li F..,Eriksen J..,Finer-Moore J..,Chang R..,Nguyen P..,...&Stroud R.M..(2020).Ion transport and regulation in a synaptic vesicle glutamate transporter.,368(6493).
MLA	Li F.,et al."Ion transport and regulation in a synaptic vesicle glutamate transporter".368.6493(2020).