气候变化领域集成服务门户(CCPortal): A noncompeting pair of human neutralizing antibodies block COVID-19 virus binding to its receptor ACE2

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DOI	10.1126/science.abc2241
	A noncompeting pair of human neutralizing antibodies block COVID-19 virus binding to its receptor ACE2
	Wu Y.; Wang F.; Shen C.; Peng W.; Li D.; Zhao C.; Li Z.; Li S.; Bi Y.; Yang Y.; Gong Y.; Xiao H.; Fan Z.; Tan S.; Wu G.; Tan W.; Lu X.; Fan C.; Wang Q.; Liu Y.; Zhang C.; Qi J.; Gao G.F.; Gao F.; Liu L.
发表日期	2020
ISSN	0036-8075
起始页码	1274
结束页码	1278
卷号	368 期号:6496
英文摘要	Neutralizing antibodies could potentially be used as antivirals against the coronavirus disease 2019 (COVID-19) pandemic. Here, we report isolation of four human-origin monoclonal antibodies from a convalescent patient, all of which display neutralization abilities. The antibodies B38 and H4 block binding between the spike glycoprotein receptor binding domain (RBD) of the virus and the cellular receptor angiotensin-converting enzyme 2 (ACE2). A competition assay indicated different epitopes on the RBD for these two antibodies, making them a potentially promising virus-targeting monoclonal antibody pair for avoiding immune escape in future clinical applications. Moreover, a therapeutic study in a mouse model validated that these antibodies can reduce virus titers in infected lungs. The RBD-B38 complex structure revealed that most residues on the epitope overlap with the RBD-ACE2 binding interface, explaining the blocking effect and neutralizing capacity. Our results highlight the promise of antibody-based therapeutics and provide a structural basis for rational vaccine design. © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
关键词	Coronavirus angiotensin converting enzyme 2 coronavirus spike glycoprotein dipeptidyl carboxypeptidase epitope monoclonal antibody neutralizing antibody virus antibody virus receptor animal chemistry Coronavirus infection disease model human immunology isolation and purification lung mouse pandemic protein domain serodiagnosis virology virus load virus pneumonia Animals Antibodies, Monoclonal Antibodies, Neutralizing Antibodies, Viral Coronavirus Infections Disease Models, Animal Humans Immunodominant Epitopes Lung Mice Neutralization Tests Pandemics Peptidyl-Dipeptidase A Pneumonia, Viral Protein Domains Receptors, Virus Spike Glycoprotein, Coronavirus Viral Load
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133441
推荐引用方式 GB/T 7714	Wu Y.,Wang F.,Shen C.,et al. A noncompeting pair of human neutralizing antibodies block COVID-19 virus binding to its receptor ACE2[J]. Science,2020,368(6496).
APA	Wu Y..,Wang F..,Shen C..,Peng W..,Li D..,...&Liu L..(2020).A noncompeting pair of human neutralizing antibodies block COVID-19 virus binding to its receptor ACE2.,368(6496).
MLA	Wu Y.,et al."A noncompeting pair of human neutralizing antibodies block COVID-19 virus binding to its receptor ACE2".368.6496(2020).