气候变化领域集成服务门户(CCPortal): Cell position fates and collective fountain flow in bacterial biofilms revealed by light-sheet microscopy

CCPortal

DOI	10.1126/science.abb8501
	Cell position fates and collective fountain flow in bacterial biofilms revealed by light-sheet microscopy
	Qin B.; Fei C.; Bridges A.A.; Mashruwala A.A.; Stone H.A.; Wingreen N.S.; Bassler B.L.
发表日期	2020
ISSN	10959203
起始页码	71
结束页码	77
卷号	369 期号:6499
英文摘要	Bacterial biofilms represent a basic form of multicellular organization that confers survival advantages to constituent cells. The sequential stages of cell ordering during biofilm development have been studied in the pathogen and model biofilm-former Vibrio cholerae It is unknown how spatial trajectories of individual cells and the collective motions of many cells drive biofilm expansion. We developed dual-view light-sheet microscopy to investigate the dynamics of biofilm development from a founder cell to a mature three-dimensional community. Tracking of individual cells revealed two distinct fates: one set of biofilm cells expanded ballistically outward, while the other became trapped at the substrate. A collective fountain-like flow transported cells to the biofilm front, bypassing members trapped at the substrate and facilitating lateral biofilm expansion. This collective flow pattern was quantitatively captured by a continuum model of biofilm growth against substrate friction. Coordinated cell movement required the matrix protein RbmA, without which cells expanded erratically. Thus, tracking cell lineages and trajectories in space and time revealed how multicellular structures form from a single founder cell. Copyright © 2020, American Association for the Advancement of Science.
关键词	bacterial protein scleroprotein biofilm cytology genetics metabolism microscopy motion mutation physiology procedures single cell analysis Vibrio cholerae Bacterial Proteins Biofilms Extracellular Matrix Proteins Microscopy Motion Mutation Single-Cell Analysis Vibrio cholerae
语种	英语
来源机构	Science (New York, N.Y.)
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133369
推荐引用方式 GB/T 7714	Qin B.,Fei C.,Bridges A.A.,et al. Cell position fates and collective fountain flow in bacterial biofilms revealed by light-sheet microscopy[J]. Science (New York, N.Y.),2020,369(6499).
APA	Qin B..,Fei C..,Bridges A.A..,Mashruwala A.A..,Stone H.A..,...&Bassler B.L..(2020).Cell position fates and collective fountain flow in bacterial biofilms revealed by light-sheet microscopy.,369(6499).
MLA	Qin B.,et al."Cell position fates and collective fountain flow in bacterial biofilms revealed by light-sheet microscopy".369.6499(2020).