气候变化领域集成服务门户(CCPortal): A phage-encoded anti-CRISPR enables complete evasion of type VI-A CRISPR-Cas immunity

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DOI	10.1126/science.abb6151
	A phage-encoded anti-CRISPR enables complete evasion of type VI-A CRISPR-Cas immunity
	Meeske A.J.; Jia N.; Cassel A.K.; Kozlova A.; Liao J.; Wiedmann M.; Patel D.J.; Marraffini L.A.
发表日期	2020
ISSN	10959203
起始页码	54
结束页码	59
卷号	369 期号:6499
英文摘要	The CRISPR RNA (crRNA)-guided nuclease Cas13 recognizes complementary viral transcripts to trigger the degradation of both host and viral RNA during the type VI CRISPR-Cas antiviral response. However, how viruses can counteract this immunity is not known. We describe a listeriaphage (ϕLS46) encoding an anti-CRISPR protein (AcrVIA1) that inactivates the type VI-A CRISPR system of Listeria seeligeri Using genetics, biochemistry, and structural biology, we found that AcrVIA1 interacts with the guide-exposed face of Cas13a, preventing access to the target RNA and the conformational changes required for nuclease activation. Unlike inhibitors of DNA-cleaving Cas nucleases, which cause limited immunosuppression and require multiple infections to bypass CRISPR defenses, a single dose of AcrVIA1 delivered by an individual virion completely dismantles type VI-A CRISPR-mediated immunity. Copyright © 2020, American Association for the Advancement of Science.
关键词	CRISPR associated protein endonuclease guide RNA viral protein virus RNA bacteriophage clustered regularly interspaced short palindromic repeat CRISPR Cas system DNA cleavage genetics Listeria metabolism RNA stability virology Bacteriophages Clustered Regularly Interspaced Short Palindromic Repeats CRISPR-Associated Proteins CRISPR-Cas Systems DNA Cleavage Endonucleases Listeria RNA Stability RNA, Guide RNA, Viral Viral Proteins
语种	英语
来源机构	Science (New York, N.Y.)
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133367
推荐引用方式 GB/T 7714	Meeske A.J.,Jia N.,Cassel A.K.,et al. A phage-encoded anti-CRISPR enables complete evasion of type VI-A CRISPR-Cas immunity[J]. Science (New York, N.Y.),2020,369(6499).
APA	Meeske A.J..,Jia N..,Cassel A.K..,Kozlova A..,Liao J..,...&Marraffini L.A..(2020).A phage-encoded anti-CRISPR enables complete evasion of type VI-A CRISPR-Cas immunity.,369(6499).
MLA	Meeske A.J.,et al."A phage-encoded anti-CRISPR enables complete evasion of type VI-A CRISPR-Cas immunity".369.6499(2020).