气候变化领域集成服务门户(CCPortal): Human CNS barrier-forming organoids with cerebrospinal fluid production

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DOI	10.1126/science.aaz5626
	Human CNS barrier-forming organoids with cerebrospinal fluid production
	Pellegrini L.; Bonfio C.; Chadwick J.; Begum F.; Skehel M.; Lancaster M.A.
发表日期	2020
ISSN	10959203
卷号	369 期号:6500
英文摘要	Cerebrospinal fluid (CSF) is a vital liquid, providing nutrients and signaling molecules and clearing out toxic by-products from the brain. The CSF is produced by the choroid plexus (ChP), a protective epithelial barrier that also prevents free entry of toxic molecules or drugs from the blood. Here, we establish human ChP organoids with a selective barrier and CSF-like fluid secretion in self-contained compartments. We show that this in vitro barrier exhibits the same selectivity to small molecules as the ChP in vivo and that ChP-CSF organoids can predict central nervous system (CNS) permeability of new compounds. The transcriptomic and proteomic signatures of ChP-CSF organoids reveal a high degree of similarity to the ChP in vivo. Finally, the intersection of single-cell transcriptomics and proteomic analysis uncovers key human CSF components produced by previously unidentified specialized epithelial subtypes. Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
关键词	blood brain barrier cell culture technique cerebrospinal fluid choroid plexus gene expression profiling human metabolism organoid physiology protein cerebrospinal fluid level proteomics single cell analysis Blood-Brain Barrier Cell Culture Techniques Cerebrospinal Fluid Cerebrospinal Fluid Proteins Choroid Plexus Gene Expression Profiling Humans Organoids Proteomics Single-Cell Analysis
语种	英语
来源机构	Science (New York, N.Y.)
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133366
推荐引用方式 GB/T 7714	Pellegrini L.,Bonfio C.,Chadwick J.,et al. Human CNS barrier-forming organoids with cerebrospinal fluid production[J]. Science (New York, N.Y.),2020,369(6500).
APA	Pellegrini L.,Bonfio C.,Chadwick J.,Begum F.,Skehel M.,&Lancaster M.A..(2020).Human CNS barrier-forming organoids with cerebrospinal fluid production.,369(6500).
MLA	Pellegrini L.,et al."Human CNS barrier-forming organoids with cerebrospinal fluid production".369.6500(2020).