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DOI | 10.1017/S147926211800045X |
Development of novel polymorphic nuclear and chloroplast microsatellite markers in coast redwood (Sequoia sempervirens) | |
Breidenbach, Natalie1; Gailing, Oliver1; Krutovsky, Konstantin, V1,2,3,4 | |
发表日期 | 2019 |
ISSN | 1479-2621 |
EISSN | 1479-263X |
卷号 | 17期号:3页码:293-297 |
英文摘要 | The range-wide genetic structure of the highly productive and valuable timber species Sequoia sempervirens (D. Don) Endl. is still insufficiently studied, although published data based on different genetic markers (nuclear and chloroplast microsatellites, AFLP, RFLP and isozymes) demonstrated relatively low population structure. However, more genetic markers are needed to increase the efficiency of population genetic studies in coast redwood. Therefore, we developed seven nuclear and five chloroplast microsatellite or simple sequence repeat (SSR) markers based on expressed sequence tags (ESTs) and the complete chloroplast genome sequence, respectively. All selected markers were tested in a range-wide sample representing trees from 16 locations. They are highly polymorphic microsatellite loci with the number of alleles ranging from 3 to 17, and the number of effective alleles from 1.1 to 2.48. Coast redwood is a hexaploid species, and its chloroplasts are paternally inherited. Therefore, the chloroplast SSR markers are especially useful for this species, because their genotyping is not affected by nuclear genome ploidy. Moreover, they showed high gene diversity for each locus within and across all populations and can be used to study range-wide population genetic structure, pollen-based gene flow and long-distance gene transfer. Coast redwood can propagate clonally, and nuclear polymorphic EST-SSRs can be used for clonal identification. They are linked with expressed genes and their variation can reflect variation in genes under selection, including those that could be potentially important for local adaptation of coast redwood considering the threat of climate change. |
WOS研究方向 | Plant Sciences ; Genetics & Heredity |
来源期刊 | PLANT GENETIC RESOURCES-CHARACTERIZATION AND UTILIZATION
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文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/98787 |
作者单位 | 1.Georg August Univ Gottingen, Dept Forest Genet & Forest Tree Breeding, Busgenweg 2, D-37077 Gottingen, Germany; 2.Russian Acad Sci, Lab Populat Genet, Vavilov Inst Gen Genet, Gubkina Str 3, Moscow 119991, Russia; 3.Siberian Fed Univ, Lab Forest Genom, Genome Res & Educ Ctr, Inst Fundamental Biol & Biotechnol, Akademgorodok 50a-2, Krasnoyarsk 660036, Russia; 4.Texas A&M Univ, Dept Ecosyst Sci & Management, College Stn, TX 77843 USA |
推荐引用方式 GB/T 7714 | Breidenbach, Natalie,Gailing, Oliver,Krutovsky, Konstantin, V. Development of novel polymorphic nuclear and chloroplast microsatellite markers in coast redwood (Sequoia sempervirens)[J],2019,17(3):293-297. |
APA | Breidenbach, Natalie,Gailing, Oliver,&Krutovsky, Konstantin, V.(2019).Development of novel polymorphic nuclear and chloroplast microsatellite markers in coast redwood (Sequoia sempervirens).PLANT GENETIC RESOURCES-CHARACTERIZATION AND UTILIZATION,17(3),293-297. |
MLA | Breidenbach, Natalie,et al."Development of novel polymorphic nuclear and chloroplast microsatellite markers in coast redwood (Sequoia sempervirens)".PLANT GENETIC RESOURCES-CHARACTERIZATION AND UTILIZATION 17.3(2019):293-297. |
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