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Estuarine environments are characterized by cyclical fluctuations in tides, with tidal shifts drastically, frequently, and acutely altering temperature, dissolved oxygen, and salinity. Despite these ecological challenges, the sheepshead minnow, Cyprinodon variegatus, seems to dominate estuarine landscapes. Here, we held sheepshead minnows to four temperature treatment groups for 1month. We then tested whether temperature exposure had an effect on acute hypoxia tolerance via oxidative stress. We measured superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities, as well as total antioxidant capacity and lipid peroxidation (LPO) damage in white muscle. We found that exposure to increasing temperatures (15, 20, 25, and 30 degrees C) for 1month led to significantly higher CTmax in sheepshead minnows. We also found that CAT activity significantly increased in the 20 and 25 degrees C temperature treatment groups, whereas it did not change between control and hypoxia trials. SOD activity was significantly higher in control groups of the 15 and 30 degrees C temperature treatment groups compared with hypoxia groups of these same temperature treatments. GPx activity was significantly lower in the 30 degrees C temperature treatment group regardless of control or hypoxia trials. Hydroxyl scavenging capacity varied across temperature treatment and control/hypoxia groups. Peroxyl scavenging capacity and LPO damage showed no significant differences across temperature treatment groups or between control and hypoxia trials.