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DOI | 10.1038/s41598-019-41305-y |
Pollens destroy respiratory epithelial cell anchors and drive alphaherpesvirus infection | |
Van Cleemput, Jolien1,2; Poelaert, Katrien C. K.1; Laval, Kathlyn2; Impens, Francis3,4,5; Van den Broeck, Wim6; Gevaert, Kris3,5; Nauwynck, Hans J.1 | |
发表日期 | 2019 |
ISSN | 2045-2322 |
卷号 | 9 |
英文摘要 | Pollens are well-known triggers of respiratory allergies and asthma. The pollen burden in today's ambient air is constantly increasing due to rising climate change and air pollution. How pollens interact with the respiratory mucosa remains largely unknown due to a lack of representative model systems. We here demonstrate how pollen proteases of Kentucky bluegrass, white birch and hazel selectively destroy integrity and anchorage of columnar respiratory epithelial cells, but not of basal cells, in both ex vivo respiratory mucosal explants and in vitro primary equine respiratory epithelial cells (EREC). In turn, this pollen protease-induced damage to respiratory epithelial cell anchorage resulted in increased infection by the host-specific and ancestral alphaherpesvirus equine herpesvirus type 1 (EHV1). Pollen proteases of all three plant species were characterized by zymography and those of white birch were fully identified for the first time as serine proteases of the subtilase family and meiotic prophase aminopeptidase 1 using mass spectrometry-based proteomics. Together, our findings demonstrate that pollen proteases selectively and irreversibly damage integrity and anchorage of columnar respiratory epithelial cells. In turn, alphaherpesviruses benefit from this partial loss-of-barrier function, resulting in increased infection of the respiratory epithelium. |
WOS研究方向 | Science & Technology - Other Topics |
来源期刊 | SCIENTIFIC REPORTS |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/95026 |
作者单位 | 1.Univ Ghent, Fac Vet Med, Dept Virol Parasitol & Immunol, Salisburylaan 133, B-9820 Merelbeke, Belgium; 2.Princeton Univ, Dept Mol Biol, 119 Lewis Thomas Lab,Washington Rd, Princeton, NJ 08544 USA; 3.VIB Ctr Med Biotechnol, Albert Baertsoenkaai 3, B-9000 Ghent, Belgium; 4.VIB Prote Core, Albert Baertsoenkaai 3, B-9000 Ghent, Belgium; 5.Univ Ghent, Dept Biomol Med, Albert Baertsoenkaai 3, B-9000 Ghent, Belgium; 6.Univ Ghent, Fac Vet Med, Dept Morphol, Salisburylaan 133, B-9820 Merelbeke, Belgium |
推荐引用方式 GB/T 7714 | Van Cleemput, Jolien,Poelaert, Katrien C. K.,Laval, Kathlyn,et al. Pollens destroy respiratory epithelial cell anchors and drive alphaherpesvirus infection[J],2019,9. |
APA | Van Cleemput, Jolien.,Poelaert, Katrien C. K..,Laval, Kathlyn.,Impens, Francis.,Van den Broeck, Wim.,...&Nauwynck, Hans J..(2019).Pollens destroy respiratory epithelial cell anchors and drive alphaherpesvirus infection.SCIENTIFIC REPORTS,9. |
MLA | Van Cleemput, Jolien,et al."Pollens destroy respiratory epithelial cell anchors and drive alphaherpesvirus infection".SCIENTIFIC REPORTS 9(2019). |
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