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DOI | 10.3389/fpls.2019.00208 |
Flavanone-3-Hydroxylase Plays an Important Role in the Biosynthesis of Spruce Phenolic Defenses Against Bark Beetles and Their Fungal Associates | |
Hammerbacher, Almuth1; Kandasamy, Dineshkumar2; Ullah, Chhana2; Schmidt, Axel2; Wright, Louwrance P.3; Gershenzon, Jonathan2 | |
发表日期 | 2019 |
ISSN | 1664-462X |
卷号 | 10 |
英文摘要 | Conifer forests worldwide are becoming increasingly vulnerable to attacks by bark beetles and their fungal associates due to the effects of global warming. Attack by the bark beetle Ips typographus and the blue-stain fungus it vectors (Endoconidiophora polonica) on Norway spruce (Picea abies) is well known to induce increased production of terpene oleoresin and polyphenolic compounds. However, it is not clear whether specific compounds are important in resisting attack. In this study, we observed a significant increase in dihydroflavonol and flavan-3-ol content after inoculating Norway spruce with the bark beetle vectored fungus. A bioassay revealed that the dihydroflavonol taxifolin and the flavan-3-ol catechin negatively affected both I. typographus and E. polonica. The biosynthesis of flavan-3-ols is well studied in Norway spruce, but little is known about dihydroflavonol formation in this species. A flavanone-3-hydroxylase (F3H) was identified that catalyzed the conversion of eriodictyol to taxifolin and was highly expressed after E. polonica infection. Down-regulating F3H gene expression by RNA interference in transgenic Norway spruce resulted in significantly lower levels of both dihydroflavonols and flavan-3-ols. Therefore F3H plays a key role in the biosynthesis of defense compounds in Norway spruce that act against the bark beetle-fungus complex. This enzyme forms a defensive product, taxifolin, which is also a metabolic precursor of another defensive product, catechin, which in turn synergizes the toxicity of taxifolin to the bark beetle associated fungus. |
WOS研究方向 | Plant Sciences |
来源期刊 | FRONTIERS IN PLANT SCIENCE
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文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/93674 |
作者单位 | 1.Univ Pretoria, Forestry & Agr Biotechnol Inst, Dept Zool & Entomol, Pretoria, South Africa; 2.Max Planck Inst Chem Ecol, Dept Biochem, Jena, Germany; 3.Zeiselhof Res Farm, Pretoria, South Africa |
推荐引用方式 GB/T 7714 | Hammerbacher, Almuth,Kandasamy, Dineshkumar,Ullah, Chhana,et al. Flavanone-3-Hydroxylase Plays an Important Role in the Biosynthesis of Spruce Phenolic Defenses Against Bark Beetles and Their Fungal Associates[J],2019,10. |
APA | Hammerbacher, Almuth,Kandasamy, Dineshkumar,Ullah, Chhana,Schmidt, Axel,Wright, Louwrance P.,&Gershenzon, Jonathan.(2019).Flavanone-3-Hydroxylase Plays an Important Role in the Biosynthesis of Spruce Phenolic Defenses Against Bark Beetles and Their Fungal Associates.FRONTIERS IN PLANT SCIENCE,10. |
MLA | Hammerbacher, Almuth,et al."Flavanone-3-Hydroxylase Plays an Important Role in the Biosynthesis of Spruce Phenolic Defenses Against Bark Beetles and Their Fungal Associates".FRONTIERS IN PLANT SCIENCE 10(2019). |
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