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DOI | 10.1038/s41598-019-39101-9 |
The germ cell marker dead end reveals alternatively spliced transcripts with dissimilar expression | |
Vasconcelos, Ana Carina Nogueira1,4; Streit Jr, Danilo Pedro2; Octavera, Anna1; Miwa, Misako1; Kabeya, Naoki3; Yoshizaki, Goro1 | |
发表日期 | 2019 |
ISSN | 2045-2322 |
卷号 | 9 |
英文摘要 | Since the late 19th century, the Amazon species Colossoma macropomum (tambaqui) has been exploited commercially and the climate change has contributed to decline in tambaqui numbers. Although germ cell cryopreservation and transplantation can help preserve the species' genetic resources semipermanently, its germ cell behavior has not been analyzed to date. In this study, we isolated the tambaqui's dead end gene (dnd) homolog (tdnd) and used it as a molecular marker for germ cells to obtain basic information essential for transplantation. The amino acid sequence showed 98% similarity and 53% identity with the zebrafish dnd. Phylogenetic analysis and the presence of consensus motifs known for dnd revealed that tdnd encodes the dnd ortholog and its transcript is detectable only in the testes and ovaries, showing a strong positive signal in oocytes and spermatogonia. The tambaqui possesses, at least, three different transcripts of tdnd which show dissimilar expression profile in undifferentiated and sexually mature animals, suggesting that they play distinct roles in germline development and they may influence the choice of donors for the cell transplantation study. |
WOS研究方向 | Science & Technology - Other Topics |
来源期刊 | SCIENTIFIC REPORTS |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/93606 |
作者单位 | 1.Tokyo Univ Marine Sci & Technol, Dept Marine Biosci, Tokyo 1088477, Japan; 2.Univ Fed Rio Grande do Sul, Dept Anim Sci, BR-91540000 Porto Alegre, RS, Brazil; 3.Univ Tokyo, Dept Aquat Biosci, Tokyo 1138654, Japan; 4.Johns Hopkins Univ, Whiting Sch Engn, Inst Nanobiotechnol, Baltimore, MD 21218 USA |
推荐引用方式 GB/T 7714 | Vasconcelos, Ana Carina Nogueira,Streit Jr, Danilo Pedro,Octavera, Anna,et al. The germ cell marker dead end reveals alternatively spliced transcripts with dissimilar expression[J],2019,9. |
APA | Vasconcelos, Ana Carina Nogueira,Streit Jr, Danilo Pedro,Octavera, Anna,Miwa, Misako,Kabeya, Naoki,&Yoshizaki, Goro.(2019).The germ cell marker dead end reveals alternatively spliced transcripts with dissimilar expression.SCIENTIFIC REPORTS,9. |
MLA | Vasconcelos, Ana Carina Nogueira,et al."The germ cell marker dead end reveals alternatively spliced transcripts with dissimilar expression".SCIENTIFIC REPORTS 9(2019). |
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