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DOI | 10.1186/s12870-019-1656-7 |
Protein analysis reveals differential accumulation of late embryogenesis abundant and storage proteins in seeds of wild and cultivated amaranth species | |
Bojorquez-Velazquez, Esau1; Barrera-Pacheco, Alberto1; Espitia-Rangel, Eduardo2; Herrera-Estrella, Alfredo3; Barba de la Rosa, Ana Paulina1 | |
发表日期 | 2019 |
ISSN | 1471-2229 |
卷号 | 19 |
英文摘要 | BackgroundAmaranth is a plant naturally resistant to various types of stresses that produces seeds of excellent nutritional quality, so amaranth is a promising system for food production. Amaranth wild relatives have survived climate changes and grow under harsh conditions, however no studies about morphological and molecular characteristics of their seeds are known. Therefore, we carried out a detailed morphological and molecular characterization of wild species A. powellii and A. hybridus, and compared them with the cultivated amaranth species A. hypochondriacus (waxy and non-waxy seeds) and A. cruentus.ResultsSeed proteins were fractionated according to their polarity properties and were analysed in one-dimensional gel electrophoresis (1-DE) followed by nano-liquid chromatography coupled to tandem mass spectrometry (nLC-MS/MS). A total of 34 differentially accumulated protein bands were detected and 105 proteins were successfully identified. Late embryogenesis abundant proteins were detected as species-specific. Oleosins and oil bodies associated proteins were observed preferentially in A. cruentus. Different isoforms of the granule-bound starch synthase I, and several paralogs of 7S and 11S globulins were also identified. The in silico structural analysis from different isoforms of 11S globulins was carried out, including new types of 11S globulin not reported so far.ConclusionsThe results provide novel information about 11S globulins and proteins related in seed protection, which could play important roles in the nutritional value and adaptive tolerance to stress in amaranth species. |
WOS研究方向 | Plant Sciences |
来源期刊 | BMC PLANT BIOLOGY
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文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/93304 |
作者单位 | 1.Inst Potosino Invest Cient & Tecnol AC, San Luis Potosi 78216, Mexico; 2.Inst Nacl Invest Forestales Agr & Pecuarias, Texcoco 56250, Estado De Mexic, Mexico; 3.CINVESTAV Irapuato, Lab Nacl Genom Biodiversidad, Guanajuato 36821, Mexico |
推荐引用方式 GB/T 7714 | Bojorquez-Velazquez, Esau,Barrera-Pacheco, Alberto,Espitia-Rangel, Eduardo,et al. Protein analysis reveals differential accumulation of late embryogenesis abundant and storage proteins in seeds of wild and cultivated amaranth species[J],2019,19. |
APA | Bojorquez-Velazquez, Esau,Barrera-Pacheco, Alberto,Espitia-Rangel, Eduardo,Herrera-Estrella, Alfredo,&Barba de la Rosa, Ana Paulina.(2019).Protein analysis reveals differential accumulation of late embryogenesis abundant and storage proteins in seeds of wild and cultivated amaranth species.BMC PLANT BIOLOGY,19. |
MLA | Bojorquez-Velazquez, Esau,et al."Protein analysis reveals differential accumulation of late embryogenesis abundant and storage proteins in seeds of wild and cultivated amaranth species".BMC PLANT BIOLOGY 19(2019). |
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