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DOI | 10.1016/j.jprot.2018.02.024 |
Global effect of the lack of inorganic polyphosphate in the extremophilic archaeon Sulfolobus solfataricus: A proteomic approach | |
Soto, Daniela F.1; Recalde, Alejandra1; Orell, Alvaro1,2; Albers, Sonja-Verena3; Paradela, Alberto4; Navarro, Claudio A.1; Jerez, Carlos A.1 | |
发表日期 | 2019 |
ISSN | 1874-3919 |
EISSN | 1876-7737 |
卷号 | 191页码:143-152 |
英文摘要 | Inorganic polyphosphates (polyP) are present in all living cells and several important functions have been described for them. They are involved in the response to stress conditions, such as nutrient depletion, oxidative stress and toxic metals amongst others. A recombinant strain of Sulfolobus solfataricus unable to accumulate polyP was designed by the overexpression of its endogenous ppx gene. The overall impact of the lack of polyP on this S. solfataricus polyP (-) strain was analyzed by using quantitative proteomics (isotope-coded protein label, ICPL). Stress-related proteins, such as peroxiredoxins and heat shock proteins, proteins involved in metabolism and several others were produced at higher levels in the ppx expression strain. The polyP deficient strain showed an increased copper sensitivity and an earlier transcriptional up-regulation of copA gene coding for the P-type copper-exporting ATPase. This implies a complementary function of both copper resistance systems. These results strongly suggests that the lack of polyP makes this hyperthermophilic archaeon more sensitive to toxic conditions, such as an exposure to metals or other harmful stimuli, emphasizing the importance of this inorganic phosphate polymers in the adaptations to live in the environmental conditions in which thermoacidophilic archaea thrive. Significance: Inorganic polyphosphate (polyP) are ubiquitous molecules with many functions in living organisms. Few studies related to these polymers have been made in archaea. The construction of a polyp deficient recombinant strain of Sulfolobus solfataricus allowed the study of the global changes in the proteome of this thermoacidophilic archaeon in the absence of polyP compared with the wild type strain. The results obtained using quantitative proteomics suggest an important participation of polyP in the oxidative stress response of the cells and as having a possible metabolic role in the cell, as previously described in bacteria. The polyp deficient strain also showed an increased copper sensitivity and an earlier transcriptional up-regulation of copA, implying a complementary role of both copper resistance systems. |
WOS研究方向 | Biochemistry & Molecular Biology |
来源期刊 | JOURNAL OF PROTEOMICS |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/92143 |
作者单位 | 1.Univ Chile, Dept Biol, Lab Mol Microbiol & Biotechnol, Fac Sci, Santiago, Chile; 2.Max Planck Inst Terr Microbiol, Marburg, Germany; 3.Univ Freiburg, Inst Biol 2 Microbiol, Mol Biol Archaea, Freiburg, Germany; 4.CNB CSIC, Prote Serv, Madrid, Spain |
推荐引用方式 GB/T 7714 | Soto, Daniela F.,Recalde, Alejandra,Orell, Alvaro,et al. Global effect of the lack of inorganic polyphosphate in the extremophilic archaeon Sulfolobus solfataricus: A proteomic approach[J],2019,191:143-152. |
APA | Soto, Daniela F..,Recalde, Alejandra.,Orell, Alvaro.,Albers, Sonja-Verena.,Paradela, Alberto.,...&Jerez, Carlos A..(2019).Global effect of the lack of inorganic polyphosphate in the extremophilic archaeon Sulfolobus solfataricus: A proteomic approach.JOURNAL OF PROTEOMICS,191,143-152. |
MLA | Soto, Daniela F.,et al."Global effect of the lack of inorganic polyphosphate in the extremophilic archaeon Sulfolobus solfataricus: A proteomic approach".JOURNAL OF PROTEOMICS 191(2019):143-152. |
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