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Dryland winter wheat in the Loess Plateau is facing a yield reduction due to a shortage of soil moisture and delayed sowing time. The field experiment was conducted at Loess Plateau in Shanxi, China from 2012 to 2015, to study the effect of subsoiling and conventional tillage and different sowing dates on the soil water storage, Nitrogen (N) accumulation, and remobilization and yield of winter wheat. The results showed that subsoiling significantly improved the soil water storage (0-300 cm soil depth) and increased the contribution of N translocation to grain N and grain yield (17-36%). Delaying sowing time had reduced the soil water storage at sowing and winter accumulated growing degree days by about 180 degrees C. The contribution of N translocation to grain yield was maximum in glume + spike followed by in leaves and minimum by stem + sheath. Moreover, there was a positive relationship between the N accumulation and translocation and the soil moisture in the 20-300 cm range. Subsoiling during the fallow period and the medium sowing date was beneficial for improving the soil water storage and increased the N translocation to grain, thereby increasing the yield of wheat, especially in a dry year.