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DOI10.1104/pp.18.00716
Adaption of Roots to Nitrogen Deficiency Revealed by 3D Quantification and Proteomic Analysis
Qin, Lu1; Walk, Thomas C.2; Han, Peipei1; Chen, Liyu3; Zhang, Sheng4; Li, Yinshui1; Hu, Xiaojia1; Xie, Lihua1; Yang, Yong5; Liu, Jiping5; Lu, Xing6; Yu, Changbing1; Tian, Jiang6; Shaff, Jon E.5; Kochian, Leon, V7; Liao, Xing1; Liao, Hong3
发表日期2019
ISSN0032-0889
EISSN1532-2548
卷号179期号:1页码:329-347
英文摘要

Rapeseed (Brassica napus) is an important oil crop worldwide. However, severe inhibition of rapeseed production often occurs in the field due to nitrogen (N) deficiency. The root system is the main organ to acquire N for plant growth, but little is known about the mechanisms underlying rapeseed root adaptions to N deficiency. Here, dynamic changes in root architectural traits of N-deficient rapeseed plants were evaluated by 3D in situ quantification. Root proteome responses to N deficiency were analyzed by the tandem mass tag-based proteomics method, and related proteins were characterized further. Under N deficiency, rapeseed roots become longer, with denser cells in the meristematic zone and larger cells in the elongation zone of root tips, and also become softer with reduced solidity. A total of 171 and 755 differentially expressed proteins were identified in short- and long-term N-deficient roots, respectively. The abundance of proteins involved in cell wall organization or biogenesis was highly enhanced, but most identified peroxidases were reduced in the N-deficient roots. Notably, peroxidase activities also were decreased, which might promote root elongation while lowering the solidity of N-deficient roots. These results were consistent with the cell wall components measured in the N-deficient roots. Further functional analysis using transgenic Arabidopsis (Arabidopsis thaliana) plants demonstrated that the two root-related differentially expressed proteins contribute to the enhanced root growth under N deficiency conditions. These results provide insights into the global changes of rapeseed root responses to N deficiency and may facilitate the development of rapeseed cultivars with high N use efficiency through root-based genetic improvements.


WOS研究方向Plant Sciences
来源期刊PLANT PHYSIOLOGY
文献类型期刊论文
条目标识符http://gcip.llas.ac.cn/handle/2XKMVOVA/90917
作者单位1.Chinese Acad Agr Sci, Oil Crops Res Inst, Key Lab Biol & Genet Improvement Oil Crops, Minist Agr, Wuhan 430062, Hubei, Peoples R China;
2.Tropotech LLC, St Louis, MO 63141 USA;
3.Fujian Agr & Forestry Univ, Root Biol Ctr, Fuzhou 350002, Fujian, Peoples R China;
4.Cornell Univ, Inst Biotechnol, New York, NY 14853 USA;
5.Cornell Univ, Robert W Holley Ctr Agr & Hlth, USDA ARS, Ithaca, NY 14853 USA;
6.South China Agr Univ, Root Biol Ctr, State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangzhou 510642, Guangdong, Peoples R China;
7.Univ Saskatchewan, Global Inst Food Secur, Saskatoon, SK S7N 4J8, Canada
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Qin, Lu,Walk, Thomas C.,Han, Peipei,et al. Adaption of Roots to Nitrogen Deficiency Revealed by 3D Quantification and Proteomic Analysis[J],2019,179(1):329-347.
APA Qin, Lu.,Walk, Thomas C..,Han, Peipei.,Chen, Liyu.,Zhang, Sheng.,...&Liao, Hong.(2019).Adaption of Roots to Nitrogen Deficiency Revealed by 3D Quantification and Proteomic Analysis.PLANT PHYSIOLOGY,179(1),329-347.
MLA Qin, Lu,et al."Adaption of Roots to Nitrogen Deficiency Revealed by 3D Quantification and Proteomic Analysis".PLANT PHYSIOLOGY 179.1(2019):329-347.
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