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DOI | 10.1146/annurev-biochem-062917-012837 |
Horizontal Cell Biology: Monitoring Global Changes of Protein Interaction States with the Proteome-Wide Cellular Thermal Shift Assay (CETSA) | |
Dai, Lingyun1; Prabhu, Nayana1; Yu, Liang Ying1,2; Bacanu, Smaranda2; Ramos, Anderson Daniel2; Nordlund, Par1,2,3 | |
发表日期 | 2019 |
ISSN | 0066-4154 |
EISSN | 1545-4509 |
卷号 | 88页码:383-408 |
英文摘要 | The cellular thermal shift assay (CETSA) is a biophysical technique allowing direct studies of ligand binding to proteins in cells and tissues. The proteome-wide implementation of CETSA with mass spectrometry detection (MS-CETSA) has now been successfully applied to discover targets for orphan clinical drugs and hits from phenotypic screens, to identify offtargets, and to explain poly-pharmacology and drug toxicity. Highly sensitive multidimensional MS-CETSA implementations can now also access binding of physiological ligands to proteins, such as metabolites, nucleic acids, and other proteins. MS-CETSA can thereby provide comprehensive information on modulations of protein interaction states in cellular processes, including downstream effects of drugs and transitions between different physiological cell states. Such horizontal information on ligand modulation in cells is largely orthogonal to vertical information on the levels of different proteins and therefore opens novel opportunities to understand operational aspects of cellular proteomes. |
WOS研究方向 | Biochemistry & Molecular Biology |
来源期刊 | ANNUAL REVIEW OF BIOCHEMISTRY, VOL 88
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文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/90743 |
作者单位 | 1.Nanyang Technol Univ, Sch Biol Sci, Singapore 138673, Singapore; 2.Karolinska Inst, Dept Pathol & Oncol, S-17177 Stockholm, Sweden; 3.ASTAR, Inst Mol & Cellular Biol, Singapore 138673, Singapore |
推荐引用方式 GB/T 7714 | Dai, Lingyun,Prabhu, Nayana,Yu, Liang Ying,et al. Horizontal Cell Biology: Monitoring Global Changes of Protein Interaction States with the Proteome-Wide Cellular Thermal Shift Assay (CETSA)[J],2019,88:383-408. |
APA | Dai, Lingyun,Prabhu, Nayana,Yu, Liang Ying,Bacanu, Smaranda,Ramos, Anderson Daniel,&Nordlund, Par.(2019).Horizontal Cell Biology: Monitoring Global Changes of Protein Interaction States with the Proteome-Wide Cellular Thermal Shift Assay (CETSA).ANNUAL REVIEW OF BIOCHEMISTRY, VOL 88,88,383-408. |
MLA | Dai, Lingyun,et al."Horizontal Cell Biology: Monitoring Global Changes of Protein Interaction States with the Proteome-Wide Cellular Thermal Shift Assay (CETSA)".ANNUAL REVIEW OF BIOCHEMISTRY, VOL 88 88(2019):383-408. |
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