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Soil respiration is one of the main fluxes in the global carbon cycle. The effect of temperature on soil respiration is well understood. The response of soil respiration to temperature warming is called apparent temperature sensitivity (Q(10)) of soil respiration, which is an important parameter in modeling soil CO2 effluxes under global climate warming. The difference of Q(10) between daytime and nighttime was hardly reported although attentions are attracted by the differences of temperature change and its effects on vegetation productivity. In this study, we investigated the Q(10) of soil respiration in daytime and nighttime by modeling empirical functions based on the in situ measurement of soil respiration and temperature in temperate and subtropical forests of eastern China. Our results showed that the Q(10) of soil respiration is higher in nighttime with the mean value of 2.74 and 2.35 than daytime with the average of 2.49 and 2.18 in all measured months and growing season, respectively. Moreover, the explanatory rate of soil temperature to soil respiration in nighttime is also higher than in daytime in each site in both all measured and growing seasons. The Q(10) and explanatory rate of soil temperature to soil respiration in nighttime is 1.08 and 1.15 times in daytime in growing season. These findings indicate that soil respiration has a bigger sensitivity to temperature in nighttime than daytime. The change of soil temperature explains more variation of soil respiration in nighttime than daytime.