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DOI | 10.1111/1462-2920.14423 |
Isotope discrimination by form IC RubisCO from Ralstonia eutropha and Rhodobacter sphaeroides, metabolically versatile members of 'Proteobacteria' from aquatic and soil habitats | |
Thomas, Phaedra J.1; Boller, Amanda J.1; Satagopan, Sriram2; Tabita, F. Robert2; Cavanaugh, Colleen M.3; Scott, Kathleen M.1 | |
发表日期 | 2019 |
ISSN | 1462-2912 |
EISSN | 1462-2920 |
卷号 | 21期号:1页码:72-80 |
英文摘要 | RubisCO, the CO2 fixing enzyme of the Calvin-Benson-Bassham (CBB) cycle, is responsible for the majority of carbon fixation on Earth. RubisCO fixes (CO2)-C-12 faster than (CO2)-C-13 resulting in C-13-depleted biomass, enabling the use of delta C-13 values to trace CBB activity in contemporary and ancient environments. Enzymatic fractionation is expressed as an epsilon value, and is routinely used in modelling, for example, the global carbon cycle and climate change, and for interpreting trophic interactions. Although values for spinach RubisCO (epsilon = similar to 29 parts per thousand) have routinely been used in such efforts, there are five different forms of RubisCO utilized by diverse photolithoautotrophs and chemolithoautotrophs and epsilon values, now known for four forms (IA, B, D and II), vary substantially with epsilon = 11 parts per thousand to 27 parts per thousand. Given the importance of epsilon values in delta C-13 evaluation, we measured enzymatic fractionation of the fifth form, form IC RubisCO, which is found widely in aquatic and terrestrial environments. Values were determined for two model organisms, the 'Proteobacteria' Ralstonia eutropha (epsilon = 19.0 parts per thousand) and Rhodobacter sphaeroides (epsilon = 22.4 parts per thousand). It is apparent from these measurements that all RubisCO forms measured to date discriminate less than commonly assumed based on spinach, and that enzyme epsilon values must be considered when interpreting and modelling variability of delta C-13 values in nature. |
WOS研究方向 | Microbiology |
来源期刊 | ENVIRONMENTAL MICROBIOLOGY |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/90649 |
作者单位 | 1.Univ S Florida, Dept Integrat Biol, Tampa, FL 33620 USA; 2.Ohio State Univ, Dept Microbiol, 484 W 12th Ave, Columbus, OH 43210 USA; 3.Harvard Univ, Dept Organism & Evolutionary Biol, Cambridge, MA 02138 USA |
推荐引用方式 GB/T 7714 | Thomas, Phaedra J.,Boller, Amanda J.,Satagopan, Sriram,et al. Isotope discrimination by form IC RubisCO from Ralstonia eutropha and Rhodobacter sphaeroides, metabolically versatile members of 'Proteobacteria' from aquatic and soil habitats[J],2019,21(1):72-80. |
APA | Thomas, Phaedra J.,Boller, Amanda J.,Satagopan, Sriram,Tabita, F. Robert,Cavanaugh, Colleen M.,&Scott, Kathleen M..(2019).Isotope discrimination by form IC RubisCO from Ralstonia eutropha and Rhodobacter sphaeroides, metabolically versatile members of 'Proteobacteria' from aquatic and soil habitats.ENVIRONMENTAL MICROBIOLOGY,21(1),72-80. |
MLA | Thomas, Phaedra J.,et al."Isotope discrimination by form IC RubisCO from Ralstonia eutropha and Rhodobacter sphaeroides, metabolically versatile members of 'Proteobacteria' from aquatic and soil habitats".ENVIRONMENTAL MICROBIOLOGY 21.1(2019):72-80. |
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