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| DOI | 10.3732/apps.1600076 |
| Development of SSR Markers for a Tibetan Medicinal Plant, Lancea tibetica (Phrymaceae), Based on RAD Sequencing | |
| Tian Z.; Zhang F.; Liu H.; Gao Q.; Chen S. | |
| 发表日期 | 2016 |
| ISSN | 21680450 |
| 卷号 | 4期号:11 |
| 英文摘要 | Premise of the study: Lancea tibetica (Phrymaceae), a Tibetan medicinal plant, is endemic to the Qinghai-Tibet Plateau. The over-exploitation of wild L. tibetica has led to the destruction of many populations. To enhance protection and management, biological research, especially population genetic studies, should be carried out on L. tibetica. Simple sequence repeat (SSR) markers of L. tibetica were developed to analyze population diversity. Methods and Results: Four thousand four hundred and forty-one SSR loci were identified for L. tibetica based on restriction-site associated DNA (RAD) sequencing on the Illumina HiSeq platform. One hundred SSR loci were arbitrarily selected for primer design, and 38 of them were successfully amplified. These markers were tested on 56 individuals from three populations of L. tibetica, and 10 markers displayed polymorphisms. The total number of alleles per locus ranged from three to eight, and observed and expected heterozygosities ranged from 0.200 to 1.000 and 0.683 to 0.879, respectively. We tested for cross-amplification of these 10 markers in the related species L. hirsuta and found that nine could be successfully amplified. Conclusions: The SSR markers characterized here are the first to be developed and tested in L. tibetica. They will be useful for future population genetic studies on L. tibetica and closely related species. © 2016 Tian et al. Published by the Botanical Society of America. |
| 英文关键词 | Lancea tibetica; Phrymaceae; population diversity; RAD sequencing; simple sequence repeat (SSR) |
| URL | https://www2.scopus.com/inward/record.uri?eid=2-s2.0-84994559814&doi=10.3732%2fapps.1600076&partnerID=40&md5=2278d42d373b4bd815fb9f2bc4455ec3 |
| 语种 | 英语 |
| 来源期刊 | Applications in Plant Sciences
 |
| 来源机构 | 中国科学院西北生态环境资源研究院 |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/77241 |
| 推荐引用方式 GB/T 7714 | Tian Z.； Zhang F.； Liu H.； Gao Q.； Chen S.. Development of SSR Markers for a Tibetan Medicinal Plant, Lancea tibetica (Phrymaceae), Based on RAD Sequencing[J]. 中国科学院西北生态环境资源研究院,2016,4(11). |
| APA | Tian Z.； Zhang F.； Liu H.； Gao Q.； Chen S..(2016).Development of SSR Markers for a Tibetan Medicinal Plant, Lancea tibetica (Phrymaceae), Based on RAD Sequencing.Applications in Plant Sciences,4(11). |
| MLA | Tian Z.； Zhang F.； Liu H.； Gao Q.； Chen S.."Development of SSR Markers for a Tibetan Medicinal Plant, Lancea tibetica (Phrymaceae), Based on RAD Sequencing".Applications in Plant Sciences 4.11(2016). |
| 条目包含的文件 | 条目无相关文件。 | |||||
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