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| Mechanistic insight into molecular catalysts during electrocatalytic reduction of oxygen and carbon dioxide | |
| 项目编号 | http://gepris.dfg.de/gepris/projekt/411742791 |
| sDr. MatthiasSchwalbe | |
| 项目主持机构 | Humboldt-Universität zu Berlin |
| 开始日期 | 2018 |
| 英文摘要 | Despiteallprogressintoxicologicalriskassessmenttherearestillsubstantialinformationdeficits,inparticularconcerningassessmentofsubtoxicandrepeatedexposurescenariosaswellasintheunderstandinginthestressresponseduetoparticleoverloadandthepotentialofadaptiveresponsetowardslowdoseexposure.Itisstillnotunderstoodwhetherthedifferencesconcerningsensitivityandvariabilitybetweenhumantissueandotherspeciesorbetweentumorcelllinesandprimarycellsaresubstantial.ThedesignedprojectaimstodeterminethemobilityandcellulareffectsofBariumsulphateandTitandioxidenanoparticle(positivecontrol)onrespiratorytissueofnativehumanlung(L-MOC)aswellason3Dco-culturesofhumanlungcellsandendothelialcells.Thebiologicalmaterialwillbeexposedtowardssubtoxicamountofnanoparticleaerosols(BaSO4,TiO2)inordertoavoidparticleoverload.Theexposurescenariowillbeappliedrepeatedlyandeffectwillbemonitoredoverseveralweeks.Theprojectwillfocusonfollowingtasks:1) Isthereanydifferencebetween3DConfigurationofcellcultureinserts(MatriGrids®)and2Dculturesystemswithrespectofdepositionofnanoparticles?Furthermoredoesthecellularresponsedifferaccordingly?2) IsthesensitivityconcerningstressreactionthesameinprimarylungcellscomparedtopermanentcelllineA549whenbothcelltypesaremaintainedin3Dco-cultureconditions.3) Doesthesensitivityofperipheral(respiratory)lungcellschangebetweenphysiologicalmorphologyinL-MOCcomparedto3Dco-cultureconfiguration?ThenanoparticleaerosolswillbeappliedassingleorrepeateddoseinanexposurechamberwithMatriGrids®cultureinserts(expertiseTILUM)onprimarylungtissue(expertiseMLU).Monitoringtimeofcellularresponseswillbeextendeduptofourweeks.Theparametersofinterestarecytotoxiceffects(viability,markerforreactiveoxygenspecies,levelofglutathione,expressionandreleaseofcytokines),markersforepithelialmorphologyandcelltocellcontacts.Thespecialfocuswillbelaidonlowsubtoxicexposurelevelsandonmonitoringofcellularreactionsoverseveralweeks.Theaimistodeterminewhicheffectsaredirectparticleassociatedandwhichareinducedbymediatorsofinflammation.Theprojectwillcombinetheexpertisefromengineeringscienceandtoxicologyinordertoestablishandcontrolconditionsofaerosolgenerationandapplicationoncellculture.TheexposurechambersandMatriGrids®3DinsertsareestablishedatTIULMandwillbeoptimizedaccordingtotheneedsoftheprojects.TheresultswillprovidethebasestoderiveNOAELforBaSO4toenableriskassessmentfornanoparticlesforinvivosituationneithertooverestimatenorunderestimate. |
| 学科分类 | 0504 - 高分子科学;05 - 化学科学 |
| 资助机构 | DE-DFG |
| 项目类型 | Research Grants |
| 国家 | DE |
| 语种 | 英语 |
| 文献类型 | 项目 |
| 条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/73286 |
| 推荐引用方式 GB/T 7714 | sDr. MatthiasSchwalbe.Mechanistic insight into molecular catalysts during electrocatalytic reduction of oxygen and carbon dioxide.2018. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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