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Alternatives to in vivo tests to detect endocrine disrupting chemicals (EDCs) in fish and amphibians - screening for estrogen, androgen and thyroid hormone disruption
Scholz, S.1; Renner, P.1; Belanger, S. E.2; Busquet, F.3; Davi, R.4; Demeneix, B. A.5; Denny, J. S.6; Leonard, M.7; McMaster, M. E.8; Villeneuve, D. L.6; Embry, M. R.9
发表日期2013
ISSN1040-8444
卷号43期号:1页码:45-72
英文摘要

Endocrine disruption is considered a highly relevant hazard for environmental risk assessment of chemicals, plant protection products, biocides and pharmaceuticals. Therefore, screening tests with a focus on interference with estrogen, androgen, and thyroid hormone pathways in fish and amphibians have been developed. However, they use a large number of animals and short-term alternatives to animal tests would be advantageous. Therefore, the status of alternative assays for endocrine disruption in fish and frogs was assessed by a detailed literature analysis. The aim was to (i) determine the strengths and limitations of alternative assays and (ii) present conclusions regarding chemical specificity, sensitivity, and correlation with in vivo data. Data from 1995 to present were collected related to the detection/testing of estrogen-, androgen-, and thyroid-active chemicals in the following test systems: cell lines, primary cells, fish/frog embryos, yeast and cell-free systems. The review shows that the majority of alternative assays measure effects directly mediated by receptor binding or resulting from interference with hormone synthesis. Other mechanisms were rarely analysed. A database was established and used for a quantitative and comparative analysis. For example, a high correlation was observed between cell-free ligand binding and cell-based reporter cell assays, between fish and frog estrogenic data and between fish embryo tests and in vivo reproductive effects. It was concluded that there is a need for a more systematic study of the predictive capacity of alternative tests and ways to reduce inter-and intra-assay variability.


英文关键词Regression;estrogens;metamorphosis;reproduction;androgens;thyroid hormones;estradiol;thyroxine;embryo;alternatives to testing of animals;in vitro;variability;dose-response;receptor binding
语种英语
WOS记录号WOS:000312958700003
来源期刊CRITICAL REVIEWS IN TOXICOLOGY
来源机构美国环保署
文献类型期刊论文
条目标识符http://gcip.llas.ac.cn/handle/2XKMVOVA/61584
作者单位1.UFZ Helmholtz Ctr Environm Res, Dept Bioanalyt Ecotoxicol, D-04318 Leipzig, Germany;
2.Procter & Gamble, Cincinnati, OH USA;
3.Johns Hopkins Univ, CAAT, Baltimore, MD USA;
4.ExxonMobil Biomedical Sci Inc, Amandale, NJ USA;
5.Museum Natl Hist Nat, F-75231 Paris, France;
6.US EPA, Midcontinent Ecol Div, Duluth, MN USA;
7.LOreal, Aulnay Sous Bois, France;
8.Environm Canada, Burlington, ON L7R 4A6, Canada;
9.ILSI Hlth & Environm Sci Inst, Washington, DC USA
推荐引用方式
GB/T 7714
Scholz, S.,Renner, P.,Belanger, S. E.,et al. Alternatives to in vivo tests to detect endocrine disrupting chemicals (EDCs) in fish and amphibians - screening for estrogen, androgen and thyroid hormone disruption[J]. 美国环保署,2013,43(1):45-72.
APA Scholz, S..,Renner, P..,Belanger, S. E..,Busquet, F..,Davi, R..,...&Embry, M. R..(2013).Alternatives to in vivo tests to detect endocrine disrupting chemicals (EDCs) in fish and amphibians - screening for estrogen, androgen and thyroid hormone disruption.CRITICAL REVIEWS IN TOXICOLOGY,43(1),45-72.
MLA Scholz, S.,et al."Alternatives to in vivo tests to detect endocrine disrupting chemicals (EDCs) in fish and amphibians - screening for estrogen, androgen and thyroid hormone disruption".CRITICAL REVIEWS IN TOXICOLOGY 43.1(2013):45-72.
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