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DOI | 10.1371/journal.pone.0085894 |
Bisphenol A Exposure Alters Developmental Gene Expression in the Fetal Rhesus Macaque Uterus | |
Calhoun, Kathryn C.1,2; Padilla-Banks, Elizabeth1; Jefferson, Wendy N.1; Liu, Liwen3; Gerrish, Kevin E.3; Young, Steven L.2; Wood, Charles E.4; Hunt, Patricia A.5; VandeVoort, Catherine A.6,7; Williams, Carmen J.1 | |
发表日期 | 2014-01-23 |
ISSN | 1932-6203 |
卷号 | 9期号:1 |
英文摘要 | Bisphenol A (BPA) exposure results in numerous developmental and functional abnormalities in reproductive organs in rodent models, but limited data are available regarding BPA effects in the primate uterus. To determine if maternal oral BPA exposure affects fetal uterine development in a non-human primate model, pregnant rhesus macaques carrying female fetuses were exposed orally to 400 mu g/kg BPA or vehicle control daily from gestation day (GD) 50-100 or GD100-165. Fetal uteri were collected at the completion of treatment (GD100 or GD165); tissue histology, cell proliferation, and expression of estrogen receptor alpha (ER alpha) and progesterone receptor (PR) were compared to that of controls. Gene expression analysis was conducted using rhesus macaque microarrays. There were no significant differences in histology or in the percentage of cells expressing the proliferation marker Ki-67, ER alpha, or PR in BPA-exposed uteri compared to controls at GD100 or GD165. Minimal differences in gene expression were observed between BPA-exposed and control GD100 uteri. However, at GD165, BPA-exposed uteri had significant differences in gene expression compared to controls. Several of the altered genes, including HOXA13, WNT4, and WNT5A, are critical for reproductive organ development and/or adult function. We conclude that second or third trimester BPA exposure does not significantly affect fetal uterus development based on morphological, proliferation, and steroid hormone receptor assessments. However, differences in expression of key developmental genes after third trimester exposure suggest that BPA could alter transcriptional signals influencing uterine function later in life. |
语种 | 英语 |
WOS记录号 | WOS:000330288000031 |
来源期刊 | PLOS ONE |
来源机构 | 美国环保署 |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/61392 |
作者单位 | 1.NIEHS, Reprod Med Grp, Lab Reprod & Dev Toxicol, Res Triangle Pk, NC 27709 USA; 2.Univ N Carolina, Dept Obstet & Gynecol, Div Reprod Endocrinol & Infertil, Chapel Hill, NC USA; 3.NIEHS, Microarray Grp, Res Triangle Pk, NC 27709 USA; 4.US EPA, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, Res Triangle Pk, NC 27711 USA; 5.Washington State Univ, Sch Mol Biosci, Pullman, WA 99164 USA; 6.Univ Calif Davis, Dept Obstet & Gynecol, Davis, CA 95616 USA; 7.Univ Calif Davis, Calif Natl Primate Res Ctr, Davis, CA 95616 USA |
推荐引用方式 GB/T 7714 | Calhoun, Kathryn C.,Padilla-Banks, Elizabeth,Jefferson, Wendy N.,et al. Bisphenol A Exposure Alters Developmental Gene Expression in the Fetal Rhesus Macaque Uterus[J]. 美国环保署,2014,9(1). |
APA | Calhoun, Kathryn C..,Padilla-Banks, Elizabeth.,Jefferson, Wendy N..,Liu, Liwen.,Gerrish, Kevin E..,...&Williams, Carmen J..(2014).Bisphenol A Exposure Alters Developmental Gene Expression in the Fetal Rhesus Macaque Uterus.PLOS ONE,9(1). |
MLA | Calhoun, Kathryn C.,et al."Bisphenol A Exposure Alters Developmental Gene Expression in the Fetal Rhesus Macaque Uterus".PLOS ONE 9.1(2014). |
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