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DOI | 10.1038/ncomms6217 |
RNAi-based functional selection identifies novel cell migration determinants dependent on PI3K and AKT pathways | |
Seo, Minchul1,2; Lee, Shinrye1,3; Kim, Jong-Heon1; Lee, Won-Ha4; Hu, Guang5; Elledge, Stephen J.6; Suk, Kyoungho1 | |
发表日期 | 2014-10-01 |
ISSN | 2041-1723 |
卷号 | 5 |
英文摘要 | Lentiviral short hairpin RNA (shRNA)-mediated genetic screening is a powerful tool for identifying loss-of-function phenotype in mammalian cells. Here, we report the identification of 91 cell migration-regulating genes using unbiased genome-wide functional genetic selection. Individual knockdown or cDNA overexpression of a set of 10 candidates reveals that most of these cell migration determinants are strongly dependent on the PI3K/PTEN/AKT pathway and on their downstream signals, such as FOXO1 and p70S6K1. ALK, one of the cell migration promoting genes, uniquely uses p55 gamma regulatory subunit of PI3K, rather than more common p85 subunit, to trigger the activation of the PI3K-AKT pathway. Our method enables the rapid and cost-effective genome-wide selection of cell migration regulators. Our results emphasize the importance of the PI3K/PTEN/AKT pathway as a point of convergence for multiple regulators of cell migration. |
语种 | 英语 |
WOS记录号 | WOS:000343982800006 |
来源期刊 | NATURE COMMUNICATIONS |
来源机构 | 美国环保署 |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/61368 |
作者单位 | 1.Kyungpook Natl Univ, Sch Med, Plus KNU Biomed Convergence Program BK21, Dept Pharmacol,Brain Sci & Engn Inst, Taegu, South Korea; 2.Dongguk Univ, Coll Med, Gyeongju, South Korea; 3.Korea Brain Res Inst, Taegu, South Korea; 4.Kyungpook Natl Univ, Sch Life Sci & Biotechnol, KNU Creat BioRes Grp, Taegu, South Korea; 5.Natl Inst Environm Hlth & Sci, Mol Carcinogenesis Lab, Res Triangle Pk, NC 27709 USA; 6.Harvard Univ, Sch Med, Brigham & Womens Hosp, Dept Genet,Howard Hughes Med Inst,Div Genet, Boston, MA 02115 USA |
推荐引用方式 GB/T 7714 | Seo, Minchul,Lee, Shinrye,Kim, Jong-Heon,et al. RNAi-based functional selection identifies novel cell migration determinants dependent on PI3K and AKT pathways[J]. 美国环保署,2014,5. |
APA | Seo, Minchul.,Lee, Shinrye.,Kim, Jong-Heon.,Lee, Won-Ha.,Hu, Guang.,...&Suk, Kyoungho.(2014).RNAi-based functional selection identifies novel cell migration determinants dependent on PI3K and AKT pathways.NATURE COMMUNICATIONS,5. |
MLA | Seo, Minchul,et al."RNAi-based functional selection identifies novel cell migration determinants dependent on PI3K and AKT pathways".NATURE COMMUNICATIONS 5(2014). |
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