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DOI | 10.1261/rna.060806.117 |
U7 snRNP is recruited to histone pre-mRNA in a FLASH-dependent manner by two separate regions of the stem-loop binding protein | |
Skrajna, Aleksandra1,2; Yang, Xiao-Cui1; Bucholc, Katarzyna2; Zhang, Jun3,5; Hall, Traci M. Tanaka3; Dadlez, Michaj2; Marzluff, William F.1,4; Dominski, Zbigniew1,4 | |
发表日期 | 2017-06-01 |
ISSN | 1355-8382 |
卷号 | 23期号:6页码:938-951 |
英文摘要 | Cleavage of histone pre-mRNAs at the 3' end requires stem-loop binding protein (SLBP) and U7 snRNP that consists of U7 snRNA and a unique Sm ring containing two U7-specific proteins: Lsm10 and Lsm11. Lsm11 interacts with FLASH and together they bring a subset of polyadenylation factors to U7 snRNP, including the CPSF73 endonuclease that cleaves histone pre-mRNA. SLBP binds to a conserved stem-loop structure upstream of the cleavage site and acts by promoting an interaction between the U7 snRNP and a sequence element located downstream from the cleavage site. We show that both human and Drosophila SLBPs stabilize U7 snRNP on histone pre-mRNA via two regions that are not directly involved in recognizing the stem-loop structure: helix B of the RNA binding domain and the C-terminal region that follows the RNA binding domain. Stabilization of U7 snRNP binding to histone pre-mRNA by SLBP requires FLASH but not the polyadenylation factors. Thus, FLASH plays two roles in 3' end processing of histone pre-mRNAs: It interacts with Lsm11 to form a docking platform for the polyadenylation factors, and it cooperates with SLBP to recruit U7 snRNP to histone pre-mRNA. |
英文关键词 | histone pre-mRNA;3' end processing;SLBP;U7 snRNP;polyadenylation factors;FLASH;Lsm11 |
语种 | 英语 |
WOS记录号 | WOS:000402224300012 |
来源期刊 | RNA |
来源机构 | 美国环保署 |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/61367 |
作者单位 | 1.Univ N Carolina, Integrat Program Biol & Genome Sci, Chapel Hill, NC 27599 USA; 2.Polish Acad Sci, Inst Biochem & Biophys, Dept Biophys, PL-02106 Warsaw, Poland; 3.Natl Inst Environm Hlth Sci, Epigenet & Stem Cell Biol Lab, NIH, Res Triangle Pk, NC 27709 USA; 4.Univ N Carolina, Dept Biochem & Biophys, Chapel Hill, NC 27599 USA; 5.Univ Alabama Birmingham, Dept Chem, Birmingham, AL 35294 USA |
推荐引用方式 GB/T 7714 | Skrajna, Aleksandra,Yang, Xiao-Cui,Bucholc, Katarzyna,et al. U7 snRNP is recruited to histone pre-mRNA in a FLASH-dependent manner by two separate regions of the stem-loop binding protein[J]. 美国环保署,2017,23(6):938-951. |
APA | Skrajna, Aleksandra.,Yang, Xiao-Cui.,Bucholc, Katarzyna.,Zhang, Jun.,Hall, Traci M. Tanaka.,...&Dominski, Zbigniew.(2017).U7 snRNP is recruited to histone pre-mRNA in a FLASH-dependent manner by two separate regions of the stem-loop binding protein.RNA,23(6),938-951. |
MLA | Skrajna, Aleksandra,et al."U7 snRNP is recruited to histone pre-mRNA in a FLASH-dependent manner by two separate regions of the stem-loop binding protein".RNA 23.6(2017):938-951. |
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