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DOI | 10.1128/AEM.03462-16 |
Corexit 9500 Enhances Oil Biodegradation and Changes Active Bacterial Community Structure of Oil-Enriched Microcosms | |
Techtmann, Stephen M.1; Zhuang, Mobing2; Campo, Pablo3; Holder, Edith4; Elk, Michael4; Hazen, Terry C.5,6; Conmy, Robyn7; Domingo, Jorge W. Santo7 | |
发表日期 | 2017-05-01 |
ISSN | 0099-2240 |
卷号 | 83期号:10 |
英文摘要 | To better understand the impacts of Corexit 9500 on the structure and activity levels of hydrocarbon-degrading microbial communities, we analyzed next-generation 16S rRNA gene sequencing libraries of hydrocarbon enrichments grown at 5 and 25 degrees C using both DNA and RNA extracts as the sequencing templates. Oil biodegradation patterns in both 5 and 25 degrees C enrichments were consistent with those reported in the literature (i.e., aliphatics were degraded faster than aromatics). Slight increases in biodegradation were observed in the presence of Corexit at both temperatures. Differences in community structure were observed between treatment conditions in the DNA-based libraries. The 25 degrees C consortia were dominated by Vibrio, Idiomarina, Marinobacter, Alcanivorax, and Thalassospira species, while the 5 degrees C consortia were dominated by several species of the genera Flavobacterium, Alcanivorax, and Oleispira. Most of these genera have been linked to hydrocarbon degradation and have been observed after oil spills. Colwellia and Cycloclasticus, known aromatic degraders, were also found in these enrichments. The addition of Corexit did not have an effect on the active bacterial community structure of the 5 degrees C consortia, while at 25 degrees C, a decrease in the relative abundance of Marinobacter was observed. At 25 degrees C, Thalassospira, Marinobacter, and Idiomarina were present at higher relative abundances in the RNA than DNA libraries, suggesting that they were active in degradation. Similarly, Oleispira was greatly stimulated by the addition of oil at 5 degrees C. IMPORTANCE While dispersants such as Corexit 9500 can be used to treat oil spills, there is still debate on the effectiveness on enhancing oil biodegradation and its potential toxic effect on oil-degrading microbial communities. The results of this study provide some insights on the microbial dynamics of hydrocarbon-degrading bacterial populations in the presence of Corexit 9500. Operational taxonomic unit (OTU) analyses indicated that several OTUs were inhibited by the addition of Corexit. Conversely, a number of OTUs were stimulated by the addition of the dispersant, many of which were identified as known hydrocarbon-degrading bacteria. The results highlight the value of using RNA-based methods to further understand the impact of dispersant on the overall activity of different hydrocarbon-degrading bacterial groups. |
英文关键词 | oil biodegradation;microbial community;Gulf of Mexico;Corexit 9500;dispersant;16S RNA;hydrocarbon |
语种 | 英语 |
WOS记录号 | WOS:000401465400021 |
来源期刊 | APPLIED AND ENVIRONMENTAL MICROBIOLOGY |
来源机构 | 美国环保署 |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/61280 |
作者单位 | 1.Michigan Technol Univ, Dept Biol Sci, Houghton, MI 49931 USA; 2.Univ Cincinnati, Dept Biomed Chem & Environm Engn, Cincinnati, OH USA; 3.Cranfield Univ, Cranfield Water Sci Inst, Cranfield, Beds, England; 4.Pegasus Inc, Cincinnati, OH USA; 5.Univ Tennessee, Dept Civil & Environm Engn, Knoxville, TN USA; 6.Univ Tennessee, Ctr Environm Biotechnol, Knoxville, TN 37932 USA; 7.US EPA, Cincinnati, OH 45268 USA |
推荐引用方式 GB/T 7714 | Techtmann, Stephen M.,Zhuang, Mobing,Campo, Pablo,et al. Corexit 9500 Enhances Oil Biodegradation and Changes Active Bacterial Community Structure of Oil-Enriched Microcosms[J]. 美国环保署,2017,83(10). |
APA | Techtmann, Stephen M..,Zhuang, Mobing.,Campo, Pablo.,Holder, Edith.,Elk, Michael.,...&Domingo, Jorge W. Santo.(2017).Corexit 9500 Enhances Oil Biodegradation and Changes Active Bacterial Community Structure of Oil-Enriched Microcosms.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,83(10). |
MLA | Techtmann, Stephen M.,et al."Corexit 9500 Enhances Oil Biodegradation and Changes Active Bacterial Community Structure of Oil-Enriched Microcosms".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 83.10(2017). |
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