Climate Change Data Portal
DOI | 10.1095/biolreprod.116.139261 |
Reactive Oxygen Stimulation of Interleukin-6 Release in the Human Trophoblast Cell Line HTR-8/SVneo by the Trichlorethylene Metabolite S-(1,2-Dichloro)-L-Cysteine | |
Hassan, Iman1,3; Kumar, Anjana M.1; Park, Hae-Ryung1,4,5; Lash, Lawrence H.2; Loch-Caruso, Rita1 | |
发表日期 | 2016-09-01 |
ISSN | 0006-3363 |
卷号 | 95期号:3 |
英文摘要 | Trichloroethylene (TCE) is a common environmental pollutant associated with adverse reproductive outcomes in humans. TCE intoxication occurs primarily through its biotransformation to bioactive metabolites, including S-(1,2-dichlorovinyl)-L-cysteine (DCVC). TCE induces oxidative stress and inflammation in the liver and kidney. Although the placenta is capable of xenobiotic metabolism and oxidative stress and inflammation in placenta have been associated with adverse pregnancy outcomes, TCE toxicity in the placenta remains poorly understood. We determined the effects of DCVC by using the human extravillous trophoblast cell line HTR-8/SVneo. Exposure to 10 and 20 mu M DCVC for 10 h increased reactive oxygen species (ROS) as measured by carboxydichlorofluorescein fluorescence. Moreover, 10 and 20 mu M DCVC increased mRNA expression and release of interleukin-6 (IL-6) after 24-h exposure, and these responses were inhibited by the cysteine conjugate beta-lyase inhibitor aminooxyacetic acid and by treatments with antioxidants (alpha-tocopherol and deferoxamine), suggesting that DCVC-stimulated IL-6 release in HTR-8/SVneo cells is dependent on beta-lyase metabolic activation and increased generation of ROS. HTR-8/SVneo cells exhibited decreased mitochondrial membrane potential at 5, 10, and 20 mu M DCVC at 5, 10, and 24 h, showing that DCVC induces mitochondrial dysfunction in HTR-8/Svneo cells. The present study demon-strates that DCVC stimulated ROS generation in the human placental cell line HTR-8/SVneo and provides new evidence of mechanistic linkage between DCVC-stimulated ROS and increase in proinflammatory cytokine IL-6. Because abnormal activation of cytokines can disrupt trophoblast functions necessary for placental development and successful pregnancy, follow-up investigations relating these findings to physiologic outcomes are warranted. |
英文关键词 | cytokines;DCVC;HTR-8/SVneo cells;human placental cells;oxidative stress;reactive oxygen species;S-(1,2-dichlorovinyl)-L-cysteine;trichloroethylene;trophoblasts |
语种 | 英语 |
WOS记录号 | WOS:000383917200019 |
来源期刊 | BIOLOGY OF REPRODUCTION |
来源机构 | 美国环保署 |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/60729 |
作者单位 | 1.Univ Michigan, Dept Environm Hlth Sci, 1415 Washington Hts, Ann Arbor, MI 48109 USA; 2.Wayne State Univ, Sch Med, Dept Pharmacol, Detroit, MI 48201 USA; 3.US EPA, Tox Assessment Div, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA; 4.Harvard TH Chan Sch Publ Hlth, Program Mol & Integrat Physiol Sci, Dept Environm Hlth, Boston, MA 02115 USA; 5.Harvard TH Chan Sch Publ Hlth, Program Mol & Integrat Physiol Sci, Dept Genet & Complex Dis, Boston, MA 02115 USA |
推荐引用方式 GB/T 7714 | Hassan, Iman,Kumar, Anjana M.,Park, Hae-Ryung,et al. Reactive Oxygen Stimulation of Interleukin-6 Release in the Human Trophoblast Cell Line HTR-8/SVneo by the Trichlorethylene Metabolite S-(1,2-Dichloro)-L-Cysteine[J]. 美国环保署,2016,95(3). |
APA | Hassan, Iman,Kumar, Anjana M.,Park, Hae-Ryung,Lash, Lawrence H.,&Loch-Caruso, Rita.(2016).Reactive Oxygen Stimulation of Interleukin-6 Release in the Human Trophoblast Cell Line HTR-8/SVneo by the Trichlorethylene Metabolite S-(1,2-Dichloro)-L-Cysteine.BIOLOGY OF REPRODUCTION,95(3). |
MLA | Hassan, Iman,et al."Reactive Oxygen Stimulation of Interleukin-6 Release in the Human Trophoblast Cell Line HTR-8/SVneo by the Trichlorethylene Metabolite S-(1,2-Dichloro)-L-Cysteine".BIOLOGY OF REPRODUCTION 95.3(2016). |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。