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DOI | 10.18632/oncotarget.4560 |
Specificity protein (Sp) transcription factors and metformin regulate expression of the long non-coding RNA HULC | |
Gandhy, Shruti U.1; Imanirad, Parisa2; Jin, Un-Ho; Nair, Vijayalekshmi2; Cheng, Yating2; Corton, J. Christopher2,3; Kim, KyoungHyun4; Safe, Stephen1,2 | |
发表日期 | 2015-09-22 |
ISSN | 1949-2553 |
卷号 | 6期号:28页码:26359-26372 |
英文摘要 | Specificity protein 1 (Sp1) transcription factor (TF) regulates expression of long non-coding RNAs (lncRNAs) in hepatocellular carcinoma (HCC) cells. RNA interference (RNAi) studies showed that among several lncRNAs expressed in HepG2, SNU-449 and SK-Hep-1 cells, highly upregulated in liver cancer (HULC) was regulated not only by Sp1 but also Sp3 and Sp4 in the three cell lines. Knockdown of Sp transcription factors and HULC by RNAi showed that they play important roles in HCC cell proliferation, survival and migration. The relative contribution of Sp1, Sp3, Sp4 and HULC on these responses in HepG2, SNU-449 and SK-Hep-1 cells were cell context-and response-dependent. In the poorly differentiated SK-Hep-1 cells, knockdown of Sp1 or HULC resulted in genomic and morphological changes, indicating that Sp1 and Sp1-regulated HULC are important for maintaining the mesenchymal phenotype in this cell line. Genomic analysis showed an inverse correlation between expression of genes after knockdown of HULC and expression of those genes in liver tumors from patients. The antidiabetic drug metformin down-regulates Sp proteins in pancreatic cancer, and similar results including decreased HULC expression were observed in HepG2, SNU-449 and SK-Hep-1 cells treated with metformin, indicating that metformin and other antineoplastic agents that target Sp proteins may have clinical applications for HCC chemotherapy. |
英文关键词 | HCC;Sp proteins;HULC;IncRNAs |
语种 | 英语 |
WOS记录号 | WOS:000363160100136 |
来源期刊 | ONCOTARGET |
来源机构 | 美国环保署 |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/60562 |
作者单位 | 1.Texas A&M Hlth Sci Ctr, Inst Biosci & Technol, Houston, TX USA; 2.Texas A&M Univ, Dept Vet Physiol & Pharmacol, College Stn, TX 77843 USA; 3.US EPA, Integrated Syst Toxicol Div, Res Triangle Pk, NC 27711 USA; 4.Univ Cincinnati, Dept Environm Hlth, Cincinnati, OH USA |
推荐引用方式 GB/T 7714 | Gandhy, Shruti U.,Imanirad, Parisa,Jin, Un-Ho,et al. Specificity protein (Sp) transcription factors and metformin regulate expression of the long non-coding RNA HULC[J]. 美国环保署,2015,6(28):26359-26372. |
APA | Gandhy, Shruti U..,Imanirad, Parisa.,Jin, Un-Ho.,Nair, Vijayalekshmi.,Cheng, Yating.,...&Safe, Stephen.(2015).Specificity protein (Sp) transcription factors and metformin regulate expression of the long non-coding RNA HULC.ONCOTARGET,6(28),26359-26372. |
MLA | Gandhy, Shruti U.,et al."Specificity protein (Sp) transcription factors and metformin regulate expression of the long non-coding RNA HULC".ONCOTARGET 6.28(2015):26359-26372. |
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