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DOI | 10.1128/AEM.01443-13 |
Shared Mycobacterium avium Genotypes Observed among Unlinked Clinical and Environmental Isolates | |
Dirac, M. Ashworth1,2; Weigel, Kris M.1,2; Yakrus, Mitchell A.3; Becker, Annie L.1,2; Chen, Hui-Ling1,2; Fridley, Gina2; Sikora, Arthur1,2; Speake, Cate1,2; Hilborn, Elizabeth D.4; Pfaller, Stacy5; Cangelosi, Gerard A.1,2 | |
发表日期 | 2013-09-01 |
ISSN | 0099-2240 |
卷号 | 79期号:18页码:5601-5607 |
英文摘要 | Our understanding of the sources of Mycobacterium avium infection is partially based on genotypic matching of pathogen isolates from cases and environmental sources. These approaches assume that genotypic identity is rare in isolates from unlinked cases or sources. To test this assumption, a high-resolution PCR-based genotyping approach, large-sequence polymorphism (LSP)-mycobacterial interspersed repetitive unit-variable-number tandem repeat (MIRU-VNTR), was selected and used to analyze clinical and environmental isolates of M. avium from geographically diverse sources. Among 127 clinical isolates from seven locations in North America, South America, and Europe, 42 genotypes were observed. Among 12 of these genotypes, matches were seen in isolates from apparently unlinked patients in two or more geographic locations. Six of the 12 were also observed in environmental isolates. A subset of these isolates was further analyzed by alternative strain genotyping methods, pulsed-field gel electrophoresis and MIRU-VNTR, which confirmed the existence of geographically dispersed strain genotypes. These results suggest that caution should be exercised in interpreting high-resolution genotypic matches as evidence for an acquisition event. |
语种 | 英语 |
WOS记录号 | WOS:000323421900021 |
来源期刊 | APPLIED AND ENVIRONMENTAL MICROBIOLOGY |
来源机构 | 美国环保署 |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/60449 |
作者单位 | 1.Univ Washington, Seattle, WA 98195 USA; 2.Seattle Biomed Res Inst, Seattle, WA 98109 USA; 3.Ctr Dis Control & Prevent, Atlanta, GA USA; 4.US EPA, Res Triangle Pk, NC 27711 USA; 5.US EPA, Cincinnati, OH 45268 USA |
推荐引用方式 GB/T 7714 | Dirac, M. Ashworth,Weigel, Kris M.,Yakrus, Mitchell A.,et al. Shared Mycobacterium avium Genotypes Observed among Unlinked Clinical and Environmental Isolates[J]. 美国环保署,2013,79(18):5601-5607. |
APA | Dirac, M. Ashworth.,Weigel, Kris M..,Yakrus, Mitchell A..,Becker, Annie L..,Chen, Hui-Ling.,...&Cangelosi, Gerard A..(2013).Shared Mycobacterium avium Genotypes Observed among Unlinked Clinical and Environmental Isolates.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,79(18),5601-5607. |
MLA | Dirac, M. Ashworth,et al."Shared Mycobacterium avium Genotypes Observed among Unlinked Clinical and Environmental Isolates".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 79.18(2013):5601-5607. |
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