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DOI | 10.1016/j.jhazmat.2018.03.030 |
Microbial degradation of Cold Lake Blend and Western Canadian select dilbits by freshwater enrichments | |
Deshpande, Ruta S.1; Sundaravadivelu, Devi1; Techtmann, Stephen2; Conmy, Robyn N.3; Domingo, Jorge W. Santo3; Campo, Pablo4 | |
发表日期 | 2018-06-15 |
ISSN | 0304-3894 |
卷号 | 352页码:111-120 |
英文摘要 | Treatability experiments were conducted to determine the biodegradation of diluted bitumen (dilbit) at 5 and 25 degrees C for 72 and 60 days, respectively. Microbial consortia obtained from the Kalamazoo River Enbridge Energy spill site were enriched on dilbit at both 5 (cryo) and 25 (meso) degrees C. On every sampling day, triplicates were sacrificed and residual hydrocarbon concentrations (alkanes and polycyclic aromatic hydrocarbons) were determined by GC-MS/MS. The composition and relative abundance of different bacterial groups were identified by 16S rRNA gene sequencing analysis. While some physicochemical differences were observed between the two dilbits, their biodegradation profiles were similar. The rates and extent of degradation were greater at 25 degrees C. Both consortia metabolized 99.9% of alkanes; however, the meso consortium was more effective at removing aromatics than the cryo consortium (97.5 vs 70%). Known hydrocarbon-degrading bacteria were present in both consortia (Pseudomonas, Rhodococcus, Hydrogenophaga, Parvibaculum, Arthrobacter, Acidovorax), although their relative abundances depended on the temperatures at which they were enriched. Regardless of the dilbit type, the microbial community structure significantly changed as a response to the diminishing hydrocarbon load. Our results demonstrate that dilbit can be effectively degraded by autochthonous microbial consortia from sites with recent exposure to dilbit contamination. |
英文关键词 | Non-conventional oil;Diluted bitumen;Dilbit;Biodegradation;Microbial community structure |
语种 | 英语 |
WOS记录号 | WOS:000431469800011 |
来源期刊 | JOURNAL OF HAZARDOUS MATERIALS |
来源机构 | 美国环保署 |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/59568 |
作者单位 | 1.Pegasus Tech Serv Inc, 46 E Hollister St, Cincinnati, OH 45219 USA; 2.Michigan Technol Univ, Dept Biol Sci, Houghton, MI 49931 USA; 3.US EPA, 26 W MLK Dr, Cincinnati, OH 45268 USA; 4.Cranfield Univ, Cranfield Water Sci Inst, Cranfield MK43 0AL, Beds, England |
推荐引用方式 GB/T 7714 | Deshpande, Ruta S.,Sundaravadivelu, Devi,Techtmann, Stephen,et al. Microbial degradation of Cold Lake Blend and Western Canadian select dilbits by freshwater enrichments[J]. 美国环保署,2018,352:111-120. |
APA | Deshpande, Ruta S.,Sundaravadivelu, Devi,Techtmann, Stephen,Conmy, Robyn N.,Domingo, Jorge W. Santo,&Campo, Pablo.(2018).Microbial degradation of Cold Lake Blend and Western Canadian select dilbits by freshwater enrichments.JOURNAL OF HAZARDOUS MATERIALS,352,111-120. |
MLA | Deshpande, Ruta S.,et al."Microbial degradation of Cold Lake Blend and Western Canadian select dilbits by freshwater enrichments".JOURNAL OF HAZARDOUS MATERIALS 352(2018):111-120. |
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