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DOI10.1111/jam.13585
Differences in staining intensities affect reported occurrences and concentrations of Giardia spp. in surface drinking water sources
Alderisio, K. A.1,2; Villegas, L. F.3; Ware, M. W.4; McDonald, L. A.1,2; Xiao, L.5; Villegas, E. N.4
发表日期2017-12-01
ISSN1364-5072
卷号123期号:6页码:1607-1613
英文摘要

Aim: USEPA Method 1623, or its equivalent, is currently used to monitor for protozoan contamination of surface drinking water sources worldwide. At least three approved staining kits used for detecting Cryptosporidium and Giardia are commercially available. This study focuses on understanding the differences among staining kits used for Method 1623.


Methods and Results: Merifluor and EasyStain labelling kits were used to monitor Cryptosporidium oocyst and Giardia cyst densities in New York City's raw surface water sources. In the year following a change to the approved staining kits for use with Method 1623, an anomaly was noted in the occurrence of Giardia cysts in New York City's raw surface water. Specifically, Merifluor-stained samples had higher Giardia cyst densities as compared with those stained with EasyStain. Side by side comparison revealed significantly lower fluorescence intensities of Giardia muris as compared with Giardia duodenalis cysts when labelled with EasyStain.


Conclusions: This study showed very poor fluorescence intensity signals by EasyStain on G. muris cysts resulting in lower cyst counts, while Merifluor, with its broader Giardia cyst staining specificity, resulted in higher cyst counts, when using Methods 1623.


Significance and Impact of the Study: These results suggest that detected Giardia cyst concentrations are dependent on the staining kits used, which can result in a more or less conservative estimation of occurrences and densities of zoonotic Giardia cysts by detecting a broader range of Giardia species/Assemblages.


英文关键词drinking water;Giardia;LT2;protozoa;water quality
语种英语
WOS记录号WOS:000415182500022
来源期刊JOURNAL OF APPLIED MICROBIOLOGY
来源机构美国环保署
文献类型期刊论文
条目标识符http://gcip.llas.ac.cn/handle/2XKMVOVA/59421
作者单位1.New York City Dept Environm Protect, Valhalla, NY USA;
2.New York City Dept Environm Protect, Kingston, NY USA;
3.CB&I Fed Serv Inc, Cincinnati, OH USA;
4.US EPA, Natl Exposure Res Lab, Cincinnati, OH 45268 USA;
5.Ctr Dis Control & Prevent, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA USA
推荐引用方式
GB/T 7714
Alderisio, K. A.,Villegas, L. F.,Ware, M. W.,et al. Differences in staining intensities affect reported occurrences and concentrations of Giardia spp. in surface drinking water sources[J]. 美国环保署,2017,123(6):1607-1613.
APA Alderisio, K. A.,Villegas, L. F.,Ware, M. W.,McDonald, L. A.,Xiao, L.,&Villegas, E. N..(2017).Differences in staining intensities affect reported occurrences and concentrations of Giardia spp. in surface drinking water sources.JOURNAL OF APPLIED MICROBIOLOGY,123(6),1607-1613.
MLA Alderisio, K. A.,et al."Differences in staining intensities affect reported occurrences and concentrations of Giardia spp. in surface drinking water sources".JOURNAL OF APPLIED MICROBIOLOGY 123.6(2017):1607-1613.
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