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DOI10.1016/j.scitotenv.2014.02.110
Dustborne and airborne Gram-positive and Gram-negative bacteria in high versus low ERMI homes
Adhikari, Atin1; Kettleson, Eric M.1; Vesper, Stephen2; Kumar, Sudhir1; Popham, David L.3; Schaffer, Christopher1; Indugula, Reshmi1; Chatterjee, Kanistha1; Allam, Karteek K.1; Grinshpun, Sergey A.1; Reponen, Tiina1
发表日期2014-06-01
ISSN0048-9697
卷号482页码:92-99
英文摘要

The study aimed at investigating Gram-positive and Gram-negative bacteria in moldy and non-moldy homes, as defined by the home's Environmental Relative Moldiness Index (ERMI) value. The ERMI values were determined from floor dust samples in 2010 and 2011 and homes were classified into low (<5) and high (>5) ERMI groups based on the average ERMI values as well as 2011 ERMI values. Dust and air samples were collected from the homes in 2011 and all samples were analyzed for Gram-positive and Gram-negative bacteria using QPCR assays, endotoxin by the LAL assay, and N-acetyl-muramic acid using HPLC. In addition, air samples were analyzed for culturable bacteria. When average ERMI values were considered, the concentration and load of Gram-positive bacteria determined with QPCR in house dust, but not air, were significantly greater in high ERMI homes than in low ERMI homes. Furthermore, the concentration of endotoxin, but not muramic acid, in the dust was significantly greater in high ERMI than in low ERMI homes. In contrast, when ERMI values of 2011 were considered, Gram-negative bacteria determined with QPCR in air, endotoxin in air, and muramic acid in dust were significantly greater in high ERMI homes. The results suggest that both short-term and long-term mold contamination in homes could be linked with the bacterial concentrations in house dust, however, only the current mold status was associated with bacterial concentrations in air. Although correlations were found between endotoxin and Gram-negative bacteria as well as between muramic acid and Gram-positive bacteria in the entire data set, diverging associations were observed between the different measures of bacteria and the home moldiness. It is likely that concentrations of cells obtained by QPCR and concentrations of cell wall components are not equivalent and represent too broad categories to understand the bacterial composition and sources of the home microbiota. (C) 2014 Elsevier B.V. All rights reserved.


英文关键词Indoor air quality;Bacteria;Mold;ERMI;Endotoxin;Muramic acid
语种英语
WOS记录号WOS:000335625100011
来源期刊SCIENCE OF THE TOTAL ENVIRONMENT
来源机构美国环保署
文献类型期刊论文
条目标识符http://gcip.llas.ac.cn/handle/2XKMVOVA/59299
作者单位1.Univ Cincinnati, Dept Environm Hlth, Cincinnati, OH 45267 USA;
2.US EPA, Natl Exposure Res Lab, Cincinnati, OH 45268 USA;
3.Virginia Tech, Dept Biol Sci, Blacksburg, VA 24061 USA
推荐引用方式
GB/T 7714
Adhikari, Atin,Kettleson, Eric M.,Vesper, Stephen,et al. Dustborne and airborne Gram-positive and Gram-negative bacteria in high versus low ERMI homes[J]. 美国环保署,2014,482:92-99.
APA Adhikari, Atin.,Kettleson, Eric M..,Vesper, Stephen.,Kumar, Sudhir.,Popham, David L..,...&Reponen, Tiina.(2014).Dustborne and airborne Gram-positive and Gram-negative bacteria in high versus low ERMI homes.SCIENCE OF THE TOTAL ENVIRONMENT,482,92-99.
MLA Adhikari, Atin,et al."Dustborne and airborne Gram-positive and Gram-negative bacteria in high versus low ERMI homes".SCIENCE OF THE TOTAL ENVIRONMENT 482(2014):92-99.
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