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DOI | 10.1016/j.funbio.2014.03.002 |
Localization and speciation of arsenic in Glomus intraradices by synchrotron radiation spectroscopic analysis | |
Gonzalez-Chavez, Ma del Carmen A.1; Miller, Bradley2; Eduardo Maldonado-Mendoza, Ignacio3; Scheckel, Kirk2; Carrillo-Gonzalez, Rogelio1 | |
发表日期 | 2014-05-01 |
ISSN | 1878-6146 |
卷号 | 118期号:5-6页码:444-452 |
英文摘要 | The protective mechanisms employed by arbuscular mycorrhizal fungi (AMP) to reduce the toxic effects of arsenic on host plants remain partially unknown. The goal of this research was identifying the in situ localization and speciation of arsenic (As) in the AM fungus Rhizophagus intraradices [formerly named Glomus intraradices] exposed to arsenate [As(V)]. By using a two-compartment in vitro fungal cultures of R. intraradices-transformed carrot roots, microspectroscopic X-ray fluorescence (mu-XRF), and microspectroscopic X-ray absorption near edge structure (mu-XANES), we observed that As(V) is absorbed after 1 h in the hyphae of AMP. Three hours after exposure a decrease in the concentration of As was noticed and after 24 and 72 h no detectable As concentrations were perceived suggesting that As taken up was pumped out from the hyphae. No As was detected within the roots or hyphae in the root compartment zone three or 45 h after exposure. This suggests a dual protective mechanism to the plant by rapidly excluding As from the fungus and preventing As translocation to the plant root. mu-XANES data showed that gradual As(V) reduction occurred in the AM hyphae between 1 and 3 h after arsenic exposure and was completed after 6 h. Principal component analysis (PCA) and linear combination fitting (LCF) of mu-XANES data showed that the dominant species after reduction of As (V) by R. intraradices extra-radical hyphal was As (III) complexed with a reduced iron(II) carbonate compound. The second most abundant As species present was As (V) iron hydroxides. The remaining As (III) compounds identified by the LCF analyses suggested these molecules were made of reduced As and S. These results increase our knowledge on the mechanism of As transport in AMP and validate our hypotheses that R. intraradices directly participates in arsenic detoxification. These fungal mechanisms may help AMP colonized plants to increase their tolerance to As at contaminated sites. Published by Elsevier Ltd on behalf of The British Mycological Society. |
英文关键词 | Arbuscular mycorrhizal fungi;Arsenate;Arsenite;Reduction;X-ray absorption near edge spectroscopy |
语种 | 英语 |
WOS记录号 | WOS:000337882700002 |
来源期刊 | FUNGAL BIOLOGY |
来源机构 | 美国环保署 |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/59117 |
作者单位 | 1.Colegio Postgrad, Montecillo 56230, Estado De Mexic, Mexico; 2.US EPA, Natl Risk Management Res Lab, Land Remediat & Pollut Control Div, Cincinnati, OH 45268 USA; 3.Inst Politecn Nacl, Ctr Interdisciplinario Invest Desarrollo Integral, IPN Unidad Sinaloa, Dept Biotecnol Agr, Mexico City, DF, Mexico |
推荐引用方式 GB/T 7714 | Gonzalez-Chavez, Ma del Carmen A.,Miller, Bradley,Eduardo Maldonado-Mendoza, Ignacio,et al. Localization and speciation of arsenic in Glomus intraradices by synchrotron radiation spectroscopic analysis[J]. 美国环保署,2014,118(5-6):444-452. |
APA | Gonzalez-Chavez, Ma del Carmen A.,Miller, Bradley,Eduardo Maldonado-Mendoza, Ignacio,Scheckel, Kirk,&Carrillo-Gonzalez, Rogelio.(2014).Localization and speciation of arsenic in Glomus intraradices by synchrotron radiation spectroscopic analysis.FUNGAL BIOLOGY,118(5-6),444-452. |
MLA | Gonzalez-Chavez, Ma del Carmen A.,et al."Localization and speciation of arsenic in Glomus intraradices by synchrotron radiation spectroscopic analysis".FUNGAL BIOLOGY 118.5-6(2014):444-452. |
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