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DOI | 10.1016/j.pbb.2012.12.010 |
Acute administration of dopaminergic drugs has differential effects on locomotion in larval zebrafish | |
Irons, T. D.1,2; Kelly, P. E.3; Hunter, D. L.2; MacPhail, R. C.4; Padilla, S.2 | |
发表日期 | 2013-02-01 |
ISSN | 0091-3057 |
卷号 | 103期号:4页码:792-813 |
英文摘要 | Altered dopaminergic signaling causes behavioral changes in mammals. In general, dopaminergic receptor agonists increase locomotor activity, while antagonists decrease locomotor activity. In order to determine if zebrafish (a model organism becoming popular in pharmacology and toxicology) respond similarly, the acute effects of drugs known to target dopaminergic receptors in mammals were assessed in zebrafish larvae. Larvae were maintained in 96-well microtiter plates (1 larva/well). Non-lethal concentrations (0.2-50 mu M) of dopaminergic agonists (apomorphine, SKF-38393, and quinpirole) and antagonists (butaclamol, SCH-23390, and haloperidol) were administered at 6 days post-fertilization (dpf). An initial experiment identified the time of peak effect of each drug (20-260 min post-dosing, depending on the drug). Locomotor activity was then assessed for 70 min in alternating light and dark at the time of peak effect for each drug to delineate dose-dependent effects. All drugs altered larval locomotion in a dose-dependent manner. Both the D1- and D2-like selective agonists (SKF-38393 and quinpirole, respectively) increased activity, while the selective antagonists (SCH-23390 and haloperidol, respectively) decreased activity. Both selective antagonists also blunted the response of the larvae to changes in lighting conditions at higher doses. The nonselective drugs had biphasic effects on locomotor activity: apomorphine increased activity at the low dose and at high doses, while butaclamol increased activity at low to intermediate doses, and decreased activity at high doses. This study demonstrates that (1) larval zebrafish locomotion can be altered by dopamine receptor agonists and antagonists, (2) receptor agonists and antagonists generally have opposite effects, and (3) drugs that target dopaminergic receptors in mammals appear, in general, to elicit similar locomotor responses in zebrafish larvae. Published by Elsevier Inc. |
英文关键词 | Dopamine;Locomotor activity;Zebrafish;Acute;Receptor |
语种 | 英语 |
WOS记录号 | WOS:000315660300014 |
来源期刊 | PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR
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来源机构 | 美国环保署 |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/59027 |
作者单位 | 1.Univ N Carolina, Curriculum Toxicol, Chapel Hill, NC 27599 USA; 2.US EPA, Integrated Syst Toxicol Div, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA; 3.Univ N Carolina, Dept Biol, Chapel Hill, NC 27599 USA; 4.US EPA, Toxicol Assessment Div, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA |
推荐引用方式 GB/T 7714 | Irons, T. D.,Kelly, P. E.,Hunter, D. L.,et al. Acute administration of dopaminergic drugs has differential effects on locomotion in larval zebrafish[J]. 美国环保署,2013,103(4):792-813. |
APA | Irons, T. D.,Kelly, P. E.,Hunter, D. L.,MacPhail, R. C.,&Padilla, S..(2013).Acute administration of dopaminergic drugs has differential effects on locomotion in larval zebrafish.PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR,103(4),792-813. |
MLA | Irons, T. D.,et al."Acute administration of dopaminergic drugs has differential effects on locomotion in larval zebrafish".PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR 103.4(2013):792-813. |
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