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DOI | 10.1007/s13213-014-0932-x |
Recent literature review of soil processing methods for recovery of Bacillus anthracis spores | |
Silvestri, Erin E.1; Perkins, Sarah D.2; Feldhake, David3; Nichols, Tonya4; Schaefer, Frank W., III1 | |
发表日期 | 2015-09-01 |
ISSN | 1590-4261 |
卷号 | 65期号:3页码:1215-1226 |
英文摘要 | Identifying virulent Bacillus anthracis within soil is a difficult task due to the number and diversity of other organisms and impeding chemical constituents within soil. Regardless of the detection assay, the initial sample must be processed efficiently to ensure that debris, chemical components, and biological impurities do not obstruct downstream analysis. Soil sample processing protocols can be divided into two general types: indirect and direct. There are two requirements for successful indirect isolation of B. anthracis from soil samples: dissociate the spores from the soil particles and physically separate the free spores from the soil particles. Adding an aqueous carrier medium to a soil sample creates a sample slurry for easier manipulation. Centrifugation, high specific gravity separation, immunomagnetic separation, filtration, and settling have been used to physically separate spores from soil. Direct processing utilizes a soil sample without first separating the spores from the bulk sample and falls under two principal types: culturing on B. anthracis selective agar and bulk DNA extraction. Direct and indirect processing steps each have associated advantages and disadvantages. The objective of this review was to consolidate information acquired from previous research, focusing primarily on data gleaned in the last decade, on the processing of soils contaminated with B. anthracis. As shown in this review, an optimized soil-processing protocol with a known recovery rate and associated confidence intervals is needed. A reliable processing protocol would allow for multiple investigators and laboratories to produce high-quality, uniform results in the event of a B. anthracis release. |
英文关键词 | Bacillus anthracis spores;Soil;Indirect processing;Direct processing |
语种 | 英语 |
WOS记录号 | WOS:000359379800002 |
来源期刊 | ANNALS OF MICROBIOLOGY
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来源机构 | 美国环保署 |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/58573 |
作者单位 | 1.US EPA, Natl Homeland Secur Res Ctr, Cincinnati, OH 45268 USA; 2.Battelle Mem Inst, Columbus, OH 43201 USA; 3.Pegasus Tech Serv Inc, Cincinnati, OH 45219 USA; 4.US EPA, Natl Homeland Secur Res Ctr, Washington, DC 20460 USA |
推荐引用方式 GB/T 7714 | Silvestri, Erin E.,Perkins, Sarah D.,Feldhake, David,et al. Recent literature review of soil processing methods for recovery of Bacillus anthracis spores[J]. 美国环保署,2015,65(3):1215-1226. |
APA | Silvestri, Erin E.,Perkins, Sarah D.,Feldhake, David,Nichols, Tonya,&Schaefer, Frank W., III.(2015).Recent literature review of soil processing methods for recovery of Bacillus anthracis spores.ANNALS OF MICROBIOLOGY,65(3),1215-1226. |
MLA | Silvestri, Erin E.,et al."Recent literature review of soil processing methods for recovery of Bacillus anthracis spores".ANNALS OF MICROBIOLOGY 65.3(2015):1215-1226. |
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