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DOI10.1007/s13213-014-0932-x
Recent literature review of soil processing methods for recovery of Bacillus anthracis spores
Silvestri, Erin E.1; Perkins, Sarah D.2; Feldhake, David3; Nichols, Tonya4; Schaefer, Frank W., III1
发表日期2015-09-01
ISSN1590-4261
卷号65期号:3页码:1215-1226
英文摘要

Identifying virulent Bacillus anthracis within soil is a difficult task due to the number and diversity of other organisms and impeding chemical constituents within soil. Regardless of the detection assay, the initial sample must be processed efficiently to ensure that debris, chemical components, and biological impurities do not obstruct downstream analysis. Soil sample processing protocols can be divided into two general types: indirect and direct. There are two requirements for successful indirect isolation of B. anthracis from soil samples: dissociate the spores from the soil particles and physically separate the free spores from the soil particles. Adding an aqueous carrier medium to a soil sample creates a sample slurry for easier manipulation. Centrifugation, high specific gravity separation, immunomagnetic separation, filtration, and settling have been used to physically separate spores from soil. Direct processing utilizes a soil sample without first separating the spores from the bulk sample and falls under two principal types: culturing on B. anthracis selective agar and bulk DNA extraction. Direct and indirect processing steps each have associated advantages and disadvantages. The objective of this review was to consolidate information acquired from previous research, focusing primarily on data gleaned in the last decade, on the processing of soils contaminated with B. anthracis. As shown in this review, an optimized soil-processing protocol with a known recovery rate and associated confidence intervals is needed. A reliable processing protocol would allow for multiple investigators and laboratories to produce high-quality, uniform results in the event of a B. anthracis release.


英文关键词Bacillus anthracis spores;Soil;Indirect processing;Direct processing
语种英语
WOS记录号WOS:000359379800002
来源期刊ANNALS OF MICROBIOLOGY
来源机构美国环保署
文献类型期刊论文
条目标识符http://gcip.llas.ac.cn/handle/2XKMVOVA/58573
作者单位1.US EPA, Natl Homeland Secur Res Ctr, Cincinnati, OH 45268 USA;
2.Battelle Mem Inst, Columbus, OH 43201 USA;
3.Pegasus Tech Serv Inc, Cincinnati, OH 45219 USA;
4.US EPA, Natl Homeland Secur Res Ctr, Washington, DC 20460 USA
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GB/T 7714
Silvestri, Erin E.,Perkins, Sarah D.,Feldhake, David,et al. Recent literature review of soil processing methods for recovery of Bacillus anthracis spores[J]. 美国环保署,2015,65(3):1215-1226.
APA Silvestri, Erin E.,Perkins, Sarah D.,Feldhake, David,Nichols, Tonya,&Schaefer, Frank W., III.(2015).Recent literature review of soil processing methods for recovery of Bacillus anthracis spores.ANNALS OF MICROBIOLOGY,65(3),1215-1226.
MLA Silvestri, Erin E.,et al."Recent literature review of soil processing methods for recovery of Bacillus anthracis spores".ANNALS OF MICROBIOLOGY 65.3(2015):1215-1226.
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