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A selective pressurized liquid extraction (SPLE) method was developed for a streamlined sample preparation/cleanup to determine Aroclors and coplanar polychlorinated biphenyls (PCBs) in soil and sediment. The SPLE was coupled with an enzyme-linked immunosorbent assay (ELISA) for an effective analytical approach for environmental monitoring. Sediment or soil samples were extracted with alumina, 10% AgNO3 in silica, and sulfuric add impregnated silica with dichloromethane at 100 degrees C and 2000 psi. The SPLE offered simultaneous extraction and cleanup of the PCBs and Aroclors, eliminating the need for a post-extraction cleanup prior to ELISA Two different ELISA methods: (1) an Aroclor ELISA and (2) a coplanar PCB ELISA were evaluated. The Aroclor ELISA utilized a polyclonal antibody (Ab) with Aroclor 1254 as the calibrant and the coplanar PCB ELISA kit used a rabbit coplanar PCB Ab with PCB-126 as the calibrant. Recoveries of Aroclor 1254 in two reference soil samples were 92 +/- 2% and 106 +/- 5% by off-line coupling of SPLE with ELISA. The average recovery of Aroclor 1254 in spiked soil and sediment samples was 92 +/- 17%. Quantitative recoveries of coplanar PCBs (107-117%) in spiked samples were obtained with the combined SPLE-ELISA The estimated method detection limit was 10 ng g(-1) for Aroclor 1254 and 125 pg g(-1) for PCB-126. Estimated sample throughput for the SPLE-ELISA was about twice that of the stepwise extraction/cleanup needed for gas chromatography (GC) or GC/mass spectrometry (MS) detection. ELISA-derived uncorrected and corrected Aroclor 1254 levels correlated well (r = 0.9973 and 0.9996) with the total Aroclor concentrations as measured by GC for samples from five different contaminated sites. ELISA-derived PCB-126 concentrations were higher than the sums of the 12 coplanar PCBs generated by GC/MS with a positive correlation (r = 0.9441). Results indicate that the SPLE-ELISA approach can be used for quantitative or qualitative analysis of PCBs in soil and sediments. To our knowledge, this is the first report of an SPLE-ELISA approach not requiring a post-extraction cleanup step for detecting Aroclors and coplanar PCBs in soil and sediment. Published by Elsevier B.V.