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DOI | 10.5740/jaoacint.12-469 |
Comparison of 3M (TM) Petrifilm (TM) Aerobic Count Plates to Standard Plating Methodology for Use with AOAC Antimicrobial Efficacy Methods 955.14, 955.15, 964.02, and 966.04 as an Alternative Enumeration Procedure: Collaborative Study | |
Nelson, Maria T.1; LaBudde, Robert A.2; Tomasino, Stephen F.3; Pines, Rebecca M.3 | |
发表日期 | 2013-07-01 |
ISSN | 1060-3271 |
卷号 | 96期号:4页码:717-722 |
英文摘要 | A multilaboratory study was conducted to determine the equivalence of the 3M (TM) Petrifilm (TM) Aerobic Count Plate and standard plating methodology for measuring viable bacteria and spores recovered from hard-surface carriers (stainless steel and porcelain), also known as "control carrier counts," used in AOAC antimicrobial efficacy test methods. Six laboratories participated in the study in which carriers inoculated with Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella enterica, and spores of Bacillus subtilis were evaluated using 3M Petrifilm Aerobic Count (AC) plates and standard plating side-by-side. The data were analyzed using a matched-pair t-test to determine the between-method effect with confidence intervals. For all test organisms pooled across all laboratories, the mean difference in log(10) concentration between the standard plate count method and 3M Petrifilm AC Plates was -0.012, with a 95% confidence interval of (-0.090, +0.066), which was well within the -0.5, +0.5 interval established as the acceptance criterion. The between-carrier SD averaged 0.139; the between-replicate SD was 0.050. The carrier reproducibility, given that a single replicate per carrier is done, was estimated to be 0.148. Although differences were seen in the final concentrations of the test organisms among laboratories, there were no statistical differences between the enumeration methods. Based on the results from this study, 3M Petrifilm AC Plates are equivalent to standard plating methodology and can be used as an alternative procedure for the enumeration of test organisms used in AOAC Methods 955.14, 955.15, 964.02, and 966.04. |
语种 | 英语 |
WOS记录号 | WOS:000322936800004 |
来源期刊 | JOURNAL OF AOAC INTERNATIONAL |
来源机构 | 美国环保署 |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/57923 |
作者单位 | 1.AOAC Int, Gaithersburg, MD 20877 USA; 2.Least Cost Formulat Ltd, Virginia Beach, VA 23464 USA; 3.US EPA, Off Pesticide Programs, Biol & Econ Anal Div, Microbiol Lab Branch,Environm Sci Ctr, Ft George G Meade, MD 20755 USA |
推荐引用方式 GB/T 7714 | Nelson, Maria T.,LaBudde, Robert A.,Tomasino, Stephen F.,et al. Comparison of 3M (TM) Petrifilm (TM) Aerobic Count Plates to Standard Plating Methodology for Use with AOAC Antimicrobial Efficacy Methods 955.14, 955.15, 964.02, and 966.04 as an Alternative Enumeration Procedure: Collaborative Study[J]. 美国环保署,2013,96(4):717-722. |
APA | Nelson, Maria T.,LaBudde, Robert A.,Tomasino, Stephen F.,&Pines, Rebecca M..(2013).Comparison of 3M (TM) Petrifilm (TM) Aerobic Count Plates to Standard Plating Methodology for Use with AOAC Antimicrobial Efficacy Methods 955.14, 955.15, 964.02, and 966.04 as an Alternative Enumeration Procedure: Collaborative Study.JOURNAL OF AOAC INTERNATIONAL,96(4),717-722. |
MLA | Nelson, Maria T.,et al."Comparison of 3M (TM) Petrifilm (TM) Aerobic Count Plates to Standard Plating Methodology for Use with AOAC Antimicrobial Efficacy Methods 955.14, 955.15, 964.02, and 966.04 as an Alternative Enumeration Procedure: Collaborative Study".JOURNAL OF AOAC INTERNATIONAL 96.4(2013):717-722. |
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