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DOI | 10.1021/es404021f |
Metabolomics for in Situ Environmental Monitoring of Surface Waters Impacted by Contaminants from Both Point and Nonpoint Sources | |
Skelton, D. M.1; Ekman, D. R.1; Martinovic-Weigelt, D.2; Ankley, G. T.3; Villeneuve, D. L.3; Teng, Q.1; Collette, T. W.1 | |
发表日期 | 2014-02-18 |
ISSN | 0013-936X |
卷号 | 48期号:4页码:2395-2403 |
英文摘要 | We investigated the efficacy of metabolomics for field-monitoring of fish exposed to wastewater treatment plant (WWTP) effluents and nonpoint sources of chemical contamination. Lab-reared male fathead minnows (Pimephales promelas, FHM) were held in mobile monitoring units and exposed on-location to surface waters upstream and downstream of the effluent point source, as well as to the actual effluent at three different WWTP sites in Minnesota. After four days of exposure, livers were collected, extracted, and analyzed by H-1 NMR spectroscopy and GC-MS to characterize responses of the hepatic metabolome. Multivariate statistical analysis revealed distinct metabolite profile changes in response to effluent exposure from each of the three WWTPs. Differences among locations (i.e., upstream, downstream, and effluent) within each of the three sites were also identified. These observed differences comport with land-use and WWTP characteristics at the study sites. For example, at one of the sites, the metabolomic analyses suggested a positive interactive response from exposure to WWTP effluent and nearby nonpoint (likely agricultural related) contamination. These findings demonstrate the utility of metabolomics as a field-based technique for monitoring the exposure of fish to impacted surface waters. |
语种 | 英语 |
WOS记录号 | WOS:000331774100037 |
来源期刊 | ENVIRONMENTAL SCIENCE & TECHNOLOGY |
来源机构 | 美国环保署 |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/57522 |
作者单位 | 1.US EPA, Natl Exposure Res Lab, Athens, GA 30605 USA; 2.Univ St Thomas, St Paul, MN 55105 USA; 3.US EPA, Natl Hlth & Environm Effects Res Lab, Duluth, MN 55804 USA |
推荐引用方式 GB/T 7714 | Skelton, D. M.,Ekman, D. R.,Martinovic-Weigelt, D.,et al. Metabolomics for in Situ Environmental Monitoring of Surface Waters Impacted by Contaminants from Both Point and Nonpoint Sources[J]. 美国环保署,2014,48(4):2395-2403. |
APA | Skelton, D. M..,Ekman, D. R..,Martinovic-Weigelt, D..,Ankley, G. T..,Villeneuve, D. L..,...&Collette, T. W..(2014).Metabolomics for in Situ Environmental Monitoring of Surface Waters Impacted by Contaminants from Both Point and Nonpoint Sources.ENVIRONMENTAL SCIENCE & TECHNOLOGY,48(4),2395-2403. |
MLA | Skelton, D. M.,et al."Metabolomics for in Situ Environmental Monitoring of Surface Waters Impacted by Contaminants from Both Point and Nonpoint Sources".ENVIRONMENTAL SCIENCE & TECHNOLOGY 48.4(2014):2395-2403. |
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