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DOI10.1016/j.marpolbul.2013.03.038
Rapid method demonstration project at four New Jersey marine beaches using real time quantitative Polymerase Chain Reaction (qPCR)
Ferretti, James A.1; Tran, Hiep V.1; Peterson, Sarah J.1; Loftin, Virginia2
发表日期2013-06-15
ISSN0025-326X
卷号71期号:1-2页码:51-63
英文摘要

Real time quantitative Polymerase Chain Reaction (qPCR) was used at four marine bathing beaches in New Jersey as part of a demonstration project to evaluate the potential for use of qPCR as part of a routine beach monitoring program. Split sample analyses for Enterococcus spp. using membrane filtration (MF) and qPCR were performed for 11 weeks during the summer of 2011 using swimming advisories based on qPCR results. Comparison of qPCR and MF results from split samples indicated that there was an 82% overall agreement rate between the two methods. Results from the qPCR tests were available by noon the same day of sample collection and swimming advisories were posted on a dedicated website. The qPCR method can be more labor intensive and requires a higher level of training to perform, however, qPCR was able to assess beach water quality in a timelier manner compared to conventional MF techniques. Published by Elsevier Ltd.


英文关键词Enterococcus;Qpcr;Beach Monitoring;Membrane filtration;Pathogens
语种英语
WOS记录号WOS:000321088200020
来源期刊MARINE POLLUTION BULLETIN
来源机构美国环保署
文献类型期刊论文
条目标识符http://gcip.llas.ac.cn/handle/2XKMVOVA/57197
作者单位1.USEPA Reg 2, Div Environm Sci & Assessment, Edison, NJ 08837 USA;
2.NJDEP Bur Marine Water Monitoring, Trenton, NJ 08625 USA
推荐引用方式
GB/T 7714
Ferretti, James A.,Tran, Hiep V.,Peterson, Sarah J.,et al. Rapid method demonstration project at four New Jersey marine beaches using real time quantitative Polymerase Chain Reaction (qPCR)[J]. 美国环保署,2013,71(1-2):51-63.
APA Ferretti, James A.,Tran, Hiep V.,Peterson, Sarah J.,&Loftin, Virginia.(2013).Rapid method demonstration project at four New Jersey marine beaches using real time quantitative Polymerase Chain Reaction (qPCR).MARINE POLLUTION BULLETIN,71(1-2),51-63.
MLA Ferretti, James A.,et al."Rapid method demonstration project at four New Jersey marine beaches using real time quantitative Polymerase Chain Reaction (qPCR)".MARINE POLLUTION BULLETIN 71.1-2(2013):51-63.
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