Climate Change Data Portal
DOI | 10.1016/j.neuro.2014.08.010 |
The cellular and genomic response of rat dopaminergic neurons (N27) to coated nanosilver | |
Chorley, Brian1; Ward, William2; Simmons, Steven O.1; Vallanat, Beena2; Veronesi, Bellina1 | |
发表日期 | 2014-12-01 |
ISSN | 0161-813X |
卷号 | 45页码:12-21 |
英文摘要 | This study examined if nanosilver (nanoAg) of different sizes and coatings were differentially toxic to oxidative stress-sensitive neurons. N27 rat dopaminergic neurons were exposed (0.5-5 ppm) to a set of nanoAg of different sizes (10 nm, 75 nm) and coatings (PVP, citrate) and their physicochemical, cellular and genomic response measured. Both coatings retained their manufactured sizes in culture media, however, the zeta potentials of both sizes of PVP-coated nanoAg were significantly less electronegative than those of their citrate-coated counterparts. Markers of oxidative stress, measured at 0.5-5 ppm exposure concentrations, indicated that caspase 3/7 activity and glutathione levels were significantly increased by both sizes of PVP-coated nanoAg and by the 75 nm citrate-coated nanoAg. Both sizes of PVP-coated nanoAg also increased intra-neuronal nitrite levels and activated ARE/NRF2, a reporter gene for the oxidative stress-protection pathway. Global gene expression on N27 neurons, exposed to 0.5 ppm for 8 h, indicated a dominant effect by PVP-coated nanoAg over citrate. The 75 nm PVP-coated material altered 196 genes that were loosely associated with mitochondrial dysfunction. In contrast, the 10 nm PVP-coated nanoAg altered 82 genes that were strongly associated with NRF2 oxidative stress pathways. Less that 20% of the affected genes were shared by both sizes of PVP-coated nanoAg. These cellular and genomic findings suggest that PVP-coated nanoAg is more bioactive than citrate-coated nanoAg.. Although both sizes of PVP-coated nanoAg altered the genomic expression of N27 neurons along oxidative stress pathways, exposure to the 75 nm nanoAg favored pathways associated with mitochondrial dysfunction, whereas the 10 nm PVP-coated nanoAg affected NRF2 neuronal protective pathways. Published by Elsevier Inc. |
英文关键词 | Nanotoxicity;Nanosilver;"Capping";Surface coating;Polyvinylpyrrolidone |
语种 | 英语 |
WOS记录号 | WOS:000346955100002 |
来源期刊 | NEUROTOXICOLOGY
![]() |
来源机构 | 美国环保署 |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/57098 |
作者单位 | 1.Natl Hlth & Environm Effects Res Lab, Integrated Syst Toxicol Div, Res Triangle Pk, NC 27711 USA; 2.US EPA, Res Genom Core, Res Triangle Pk, NC 27711 USA |
推荐引用方式 GB/T 7714 | Chorley, Brian,Ward, William,Simmons, Steven O.,et al. The cellular and genomic response of rat dopaminergic neurons (N27) to coated nanosilver[J]. 美国环保署,2014,45:12-21. |
APA | Chorley, Brian,Ward, William,Simmons, Steven O.,Vallanat, Beena,&Veronesi, Bellina.(2014).The cellular and genomic response of rat dopaminergic neurons (N27) to coated nanosilver.NEUROTOXICOLOGY,45,12-21. |
MLA | Chorley, Brian,et al."The cellular and genomic response of rat dopaminergic neurons (N27) to coated nanosilver".NEUROTOXICOLOGY 45(2014):12-21. |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。