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DOI | 10.1016/j.talanta.2013.09.051 |
Evaluation of micro-colorimetric lipid determination method with samples prepared using sonication and accelerated solvent extraction methods | |
Billa, Nanditha; Hubin-Barrows, Dylan; Lahren, Tylor; Burkhard, Lawrence P. | |
发表日期 | 2014-02-15 |
ISSN | 0039-9140 |
卷号 | 119页码:620-622 |
英文摘要 | Two common laboratory extraction techniques were evaluated for routine use with the micro-colorimetric lipid determination method developed by Van Handel (1985) [2] and recently validated for small samples by Inouye and Lotufo (2006) [1]. With the accelerated solvent extraction method using chloroform: methanol solvent and the colorimetric lipid determination method, 28 of 30 samples had significant proportional bias (alpha=1%, determined using standard additions) and 1 of 30 samples had significant constant bias (alpha= 1%, determined using Youden Blank measurements). With sonic extraction, 0 of 6 samples had significant proportional bias (alpha= 1%) and 1 of 6 samples had significant constant bias (alpha=1%). These demonstrate that the accelerated solvent extraction method with chloroform:methanol solvent system creates an interference with the colorimetric assay method, and without accounting for the bias in the analysis, inaccurate measurements would be obtained. Published by Elsevier B.V. |
英文关键词 | Lipid measurement;Micro-colorimetric;Accelerated solvent extraction |
语种 | 英语 |
WOS记录号 | WOS:000331670700088 |
来源期刊 | TALANTA
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来源机构 | 美国环保署 |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/56757 |
作者单位 | US EPA, Midcontinent Ecol Div, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, Duluth, MN 55804 USA |
推荐引用方式 GB/T 7714 | Billa, Nanditha,Hubin-Barrows, Dylan,Lahren, Tylor,et al. Evaluation of micro-colorimetric lipid determination method with samples prepared using sonication and accelerated solvent extraction methods[J]. 美国环保署,2014,119:620-622. |
APA | Billa, Nanditha,Hubin-Barrows, Dylan,Lahren, Tylor,&Burkhard, Lawrence P..(2014).Evaluation of micro-colorimetric lipid determination method with samples prepared using sonication and accelerated solvent extraction methods.TALANTA,119,620-622. |
MLA | Billa, Nanditha,et al."Evaluation of micro-colorimetric lipid determination method with samples prepared using sonication and accelerated solvent extraction methods".TALANTA 119(2014):620-622. |
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